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Cited by 3Carcinogens and Genotoxicity AssessmentOral Health Pathology and TreatmentDNA Repair Mechanisms

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Detection of DNA adducts by 32P postlabeling following chronic exposure of rats to snuff

Smith, Raymond A, Lawson, Terence, Johansson, Sonny L·1997·Cancer Letters
DOI 10.1016/s0304-3835(97)00320-0
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Abstract

A surgically created canal was used as a reservoir for instillation of snuff into the lower lip of rats for 10 weeks. DNA was isolated and digested with micrococcal nuclease/spleen phosphodiesterase. Polar DNA adducts were detected in all tissues examined when digests were fractionated by high performance liquid chromatography (HPLC), 32P postlabeled, 3' dephosphorylated and analyzed by two-dimensional thin layer chromatography (TLC). DNA adducts derived from aromatic carcinogens were not detected when digests were 32P postlabeled and analyzed by four-dimensional thin layer chromatography. These results suggest that non-aromatic agents initiate carcinogenesis following exposure to snuff. The adduction to DNA in organs of the gastrointestinal tract and the kidneys indicates that snuff usage results in systemic exposure to carcinogens and may contribute to the incidence of neoplasms in organs outside the oral cavity.

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Research works printed but not linked: 25

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Metadata

Authors
Smith, R. A., Lawson, T., & Johansson, S. L.
Affiliations
University of Nebraska Medical Center (US)
Corresponding author
Smith, Raymond A
Venue
Cancer Letters
Publisher
Elsevier BV
Published
1997-12
Volume
121
Issue
1
Pages
11-17
ISSN
0304-3835 (ISSN-L)
DOI
10.1016/s0304-3835(97)00320-0
OpenAlex
W2010328881
Cited by
3
References
0
Citations by year
2026: 1 · 2025: 1 · 2023: 1
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Cancer Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
University of Kentucky
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Health Sciences › Dentistry › General Dentistry › Oral Health Pathology and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
snuff, smokeless tobacco, DNA adducts, 32P-postlabeling, long-term exposure, oral carcinogenesis, gastrointestinal tract, kidney, oral cancer
MeSH terms
Animals, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Epithelium, Kidney, Liver, Male, Nasal Cavity, Palate, Phosphorus Radioisotopes, Plants, Toxic, Stomach, Tobacco, Smokeless, Rats, Sprague-Dawley, DNA Adducts, Rats

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