Magnetic resonance lipid signals in rat brain after experimental stroke correlate with neutral lipid accumulation
Abstract
Proton magnetic resonance spectroscopy (MRS) signals from lipids in brain have been observed to increase after ischemic brain injury. However, neither the chemical identity nor the cellular location of these lipids has been established. The aim of the present study was to identify the origin of MRS lipid signals in rat brain after temporary (90 min) middle cerebral artery occlusion (MCAO). Fatty acyl proton signals were detected by short-echo one and two dimensional (1)H MRS in superfused brain slices from the infarcted hemisphere 1-5 days after MCAO. The intensities of these signals were strongly correlated with the amount of triacylglyceride and cholesterol ester in lipid extracts from the samples (r(2)=0.96, P<0.05) and were not correlated with the amount of free fatty acids in the tissue. Histological staining of tissue revealed the presence of neutral lipid droplets in infarcted regions. Dual labeling by immunohistochemistry demonstrated that these droplets were localized to microglia/macrophage (OX-42-labeled cells). These results strongly suggest that (1)H MRS lipid signals from brain after stroke arise from microglia/macrophage phagocytosis of cellular membranes.
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Ischemia is correlated with both increased lipid peroxidation and neutral lipid accumulation in the brain.
“Ischemia is correlated with both increased lipid peroxidation and neutral lipid accumulation in the brain (Yoshida et al., 1982; Gasparovic et al., 2001; Ioannou et al., 2019).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Gasparovic et al., 2001
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APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size
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- Authors
- Gasparovic, C., Rosenberg, G. A., Wallace, J. A., Estrada, E. Y., Roberts, K., Pastuszyn, A., Ahmed, W., & Graham, G. D.
- Affiliations
- University of New Mexico (US)
- Corresponding author
- Gasparovic, Charles
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2001-03
- Volume
- 301
- Issue
- 2
- Pages
- 87-90
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2110581704
- Cited by
- 67
- References
- 0
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 5 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (R01 NS21169-12, R01NS021169)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- 1H NMR, ischemic stroke, middle cerebral artery occlusion, neutral lipid accumulation, triglycerides, cholesterol esters, microglia/macrophages, OX-42, lipid droplets, phagocytosis, membrane lipids
- MeSH terms
- Animals, Brain Chemistry, Cerebral Infarction, Cholesterol Esters, Magnetic Resonance Spectroscopy, Protons, Triglycerides, Biomarkers, Rats, Wistar, Stroke, Rats
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