Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells
Abstract
As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.
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The presence of lipids leads to alterations in the binding properties of apoE with Aβ as well as other proteins.
“Since considerable conformational differences exist between lipid-free and lipid-bound forms of apoE (Blacklow, 2007), the presence of lipids leads to alterations in the binding properties of apoE with Aβ as well as other proteins (Dergunov et al., 2000; Ljungberg et al., 2003).”
From The role of apolipoprotein E in Alzheimer's disease (2009) · cites this paper as Ljungberg et al., 2003
Cites
Apolipoprotein E Is Resistant to Intracellular Degradation in Vitro and in Vivo
Rensen, P. C. N., Jong, M. C., van Vark, L. C., van der Boom, H., Hendriks, W. L., van Berkel, T. J. C., Biessen, E. A. L., & Havekes, L. M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.12.8564 · Cited by 86 · see citing works
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The role of apolipoprotein E in Alzheimer's disease
Kim, J., Basak, J. M., & Holtzman, D. M. (2009) · Neuron · doi:10.1016/j.neuron.2009.06.026 · Cited by 1,661 · see citing works
- Authors
- Ljungberg, M. C., Asuni, A., Pearce, J., Dayanandan, R., März, W., Hoffmann, M. M., Bertrand, P., Siest, G., Rupniak, H. T. R., Anderton, B. H., Huettinger, M., & Lovestone, S.
- Affiliations
- King's College London (GB)
- University of Freiburg (DE)
- Centre de Médecine Préventive (FR)
- Université Henri Poincaré (FR)
- GlaxoSmithKline (United Kingdom) (GB)
- University of Vienna (AT)
- Corresponding author
- Lovestone, S
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2003-04
- Volume
- 341
- Issue
- 1
- Pages
- 69-73
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2092846997
- Cited by
- 2
- References
- 1
- Citations by year
- 2014: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Research Portal (King's College London) (submittedVersion)
- Funders
- University of Oxford
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- apolipoprotein E, cellular uptake, LDL receptor, vesicular uptake, lysosomes, intracellular trafficking, live-cell imaging, Alzheimer's disease
- MeSH terms
- Animals, Apolipoproteins E, Cell Line, Chlorocebus aethiops, Escherichia coli, Humans, Rabbits, Recombinant Proteins, Tumor Cells, Cultured, COS Cells, Protein Isoforms, Mice
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