Tangential synaptic distribution of NMDA and AMPA receptors in rat neocortex
Abstract
We performed an electron microscopic study in layers II-III of S-1 in rats, using postembedding immunogold histochemistry to compare the synaptic distribution of N-methyl D-aspartate (NMDA) receptors (assessed with an antibody for the NMDAR1 subunit) with that of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (assessed with an antibody for the GluR2/3 subunit). Labeling for each receptor was concentrated at active zones of asymmetric synapses. Analysis of the tangential position of gold particles along the postsynaptic active zone revealed that NMDA receptors were at highest concentration in the middle of the synaptic apposition, whereas AMPA receptors were concentrated in an annulus away from its center. These data support the view that the two types of receptors are anchored by distinct subsynaptic assemblies, and raise the possibility of independent synaptic microdomains.
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NMDARs tend to be concentrated in the middle of the PSD with little overlap with AMPARs.
“NMDARs tend to be concentrated in the middle of the PSD with little overlap with AMPARs (253–255).”
From Synaptic memory and CaMKII (2023) · cites this paper as [255]
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- Authors
- Kharazia, V. N., & Weinberg, R. J.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Weinberg, R.J
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1997-11
- Volume
- 238
- Issue
- 1-2
- Pages
- 41-44
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2089078464
- Cited by
- 104
- References
- 20
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 6
- FWCI
- 2.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns029879-08)
- National Institute of Neurological Disorders and Stroke (NS-29879)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Keywords
- NMDA receptor, AMPA receptors, synaptic distribution, asymmetric synapses, postsynaptic density
- MeSH terms
- Animals, Immunohistochemistry, Male, Microscopy, Electron, Synaptic Membranes, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Receptors, AMPA, Neocortex, Rats
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