Histochemical differentiation of Dirofilaria immitis, Dirofilaria repens and Acanthocheilonema dracunculoides microfilariae by staining with a commercial kit, Leucognost-SP®
Abstract
The diagnosis of canine heartworm infection is based upon the presence of circulating Dirofilaria immitis microfilariae or on techniques for the detection of serum antibodies or antigens. In the first of these, discrimination between D. immitis, D. repens and Acanthocheilonema dracunculoides microfilariae is based upon the acid phosphatase histochemical stain. In this paper, we propose an alternative technique for histochemical staining using a commercial kit test of naphthol-AS-OL (Leucognost-SP). This offers the advantages of speed and simplicity as compared to the standard Barka procedure.
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Acid phosphatase activity offers an easily observable and reliable method for differentiation of microfilariae.
“The acid phosphatase activity offers an easily observable and reliable method for differentiation of microfilariae [36, 49].”
From Prevalence of canine heartworm ( <i>Dirofilaria immitis</i> ) disease in dogs of central Portugal (2014) · cites this paper as [36]
The low sensitivity of commercial serological kits could explain the result of five positive dogs (6%) that were microfilariae-positive and antigen-negative.
“This could explain the result of five positive dogs (6%) that were microfilariae-positive and antigen-negative [35–37].”
From Prevalence of canine heartworm ( <i>Dirofilaria immitis</i> ) disease in dogs of central Portugal (2014) · cites this paper as [36]
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References 4 · Cited by 43
- Authors
- Peribáñez, M. A., Lucientes, J., Arce, S., Morales, M., Castillo, J. A., & Gracia, M. J.
- Affiliations
- Universidad de Zaragoza (ES)
- Corresponding author
- Peribáñez, Miguel A.
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2001-12
- Volume
- 102
- Issue
- 1-2
- Pages
- 173-175
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2089792488
- Cited by
- 43
- References
- 4
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 4 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Keywords
- Dirofilaria immitis, Dirofilaria repens, heartworm infection, microfilariae, canine heartworm disease, commercial diagnostic kits
- MeSH terms
- Acid Phosphatase, Animals, Diagnosis, Differential, Dipetalonema, Dipetalonema Infections, Dirofilaria, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Histocytochemistry, Microfilariae, Reagent Kits, Diagnostic
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