High-performance tangential flow filtration using charged membranes

A model for light distribution and average solar irradiance inside outdoor tubular photobioreactors for the microalgal mass culture

Acién, F. G., García-Camacho, F., Pérez, J. A. S., Fernández-Sevilla, J. M., & Grima, E. M. (1997) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19970905)55:5 · Cited by 250

Effect of solution pH and ionic strength on the separation of albumin from immunoglobulins (IgG) by selective filtration

Saksena, S., & Zydney, A. L. (1994) · Biotechnology and Bioengineering · doi:10.1002/bit.260431009 · Cited by 227

High performance tangential flow filtration

van Reis, R., Gadam, S. D., Frautschy, L. N., Orlando, S., Goodrich, E. M., Saksena, S., Kuriyel, R., Simpson, C. M., Pearl, S., & Zydney, A. L. (1997) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19971005)56:1 · Cited by 185

Streaming potential as a tool in the characterization of ultrafiltration membranes

Nyström, M., Lindström, M., & Matthiasson, E. (1989) · Colloids and Surfaces · doi:10.1016/0166-6622(89)80245-8 · Cited by 170

Separation of proteins by charged ultrafiltration membranes

Nakao, S., Osada, H., Kurata, H., Tsuru, T., & Kimura, S. (1988) · Desalination · doi:10.1016/0011-9164(88)85054-9 · Cited by 164

Protein fractionation using electrostatic interactions in membranel filtration

van Eijndhoven, R. H. C. M., Saksena, S., & Zydney, A. L. (1995) · Biotechnology and Bioengineering · doi:10.1002/bit.260480413 · Cited by 114

Electrostatic effects on protein partitioning in size-exclusion chromatography and membrane ultrafiltration

Pujar, N. S., & Zydney, A. L. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)01003-0 · Cited by 104

Charge Regulation and Electrostatic Interactions for a Spherical Particle in a Cylindrical Pore

Pujar, N. S., & Zydney, A. L. (1997) · Journal of Colloid and Interface Science · doi:10.1006/jcis.1997.5017 · Cited by 83

Loose ultrafiltration of proteins using hydrolyzed polyacrylonitrile hollow fiber

Yang, M. (1997) · Journal of Membrane Science · doi:10.1016/s0376-7388(97)00038-0 · Cited by 78

Optimization diagram for membrane separations

van Reis, R., & Saksena, S. (1997) · Journal of Membrane Science · doi:10.1016/s0376-7388(96)00319-5 · Cited by 76

Fractionation of pasteurized skim milk proteins by dynamic filtration

Espina, V., Jaffrin, M. Y., Ding, L., & Cancino, B. (2010) · Food Research International · doi:10.1016/j.foodres.2010.03.023 · Cited by 17

Effects of protein–protein interaction in ultrafiltration based fractionation processes

Filipe, C. D. M., & Ghosh, R. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20568 · Cited by 17

Probing the change of enzymatic activity of horseradish peroxidase induced by membrane permeation using tryptophan fluorescence

Portugal, C. A. M., Lima, J. C., & Crespo, J. G. (2006) · Journal of Membrane Science · doi:10.1016/j.memsci.2006.07.053 · Cited by 16

Numerical study of BSA ultrafiltration in the limiting flux regime — Effect of variable physical properties

Afonso, A. M., Miranda, J. M., & Campos, J. B. L. M. (2009) · Desalination · doi:10.1016/j.desal.2009.05.012 · Cited by 15

1.5 State-of-the-Art and Perspectives of Organic Materials for Membrane Preparation

Ulbricht, M. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.12252-8 · Cited by 15

Exploration of ionic modification in dual‐layer hollow fiber membranes for long‐term high‐performance protein separation

Li, Y., Soh, S. C., Chung, T., & Chan, S. Y. (2008) · AIChE Journal · doi:10.1002/aic.11671 · Cited by 15

Preparation of a Low-Protein-Fouling and High-Protein-Retention Membrane via Novel Pre-Hydrolysis Treatment of Polyacrylonitrile (PAN)

Xu, D. Y., Pan, G., Ge, Y., & Yang, X. (2023) · Membranes · doi:10.3390/membranes13030310 · Cited by 14

Purification of Minute Virus of Mice using high performance tangential flow filtration

Hensgen, M. I., Czermak, P., Carlson, J. O., & Wickramasinghe, S. R. (2009) · Desalination · doi:10.1016/j.desal.2009.09.122 · Cited by 14

Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes

Zimmermanna, R., Osaki, T., Gauglitz, G., & Werner, C. (2007) · Biointerphases · doi:10.1116/1.2814066 · Cited by 14

Ultrafiltration: Fundamentals and Engineering

Lutz, H. (2010) · Elsevier eBooks · doi:10.1016/b978-0-08-093250-7.00037-2 · Cited by 14

Mammalian Expression of Isotopically Labeled Proteins for NMR Spectroscopy

Sastry, M., Bewley, C. A., & Kwong, P. D. (2012) · Advances in experimental medicine and biology · doi:10.1007/978-94-007-4954-2_11 · Cited by 14

Rejection amplification in the ultrafiltration of binary protein mixtures using sandwich configurations

Yunos, K. F. M., & Field, R. W. (2007) · Chemical Engineering and Processing - Process Intensification · doi:10.1016/j.cep.2007.07.020 · Cited by 14

Protein charge ladders: a new technique for studying electrostatic interactions in ultrafiltration systems

Menon, M. (2001) · Journal of Membrane Science · doi:10.1016/s0376-7388(00)00536-6 · Cited by 14

Retention of modified BSA by ultrafiltration membranes

Levitsky, I., Dahan, Y., Arkhangelsky, E., & Gitis, V. (2014) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.4588 · Cited by 13

Preparation and characterization of poly (methyl methacrylate) and sulfonated poly (ether ether ketone) blend ultrafiltration membranes for protein separation applications

Arthanareeswaran, G., Thanikaivelan, P., & Raajenthiren, M. (2008) · Materials Science and Engineering C · doi:10.1016/j.msec.2008.06.015 · Cited by 13

Purification of transgenic tobacco-derived recombinant human monoclonal antibody

Mayani, M., Filipe, C. D. M., McLean, M. D., Hall, J. C., & Ghosh, R. (2012) · Biochemical Engineering Journal · doi:10.1016/j.bej.2012.12.007 · Cited by 13

Use of protein charge ladders to study electrostatic interactions during protein ultrafiltration

Ebersold, M. F., & Zydney, A. L. (2003) · Biotechnology and Bioengineering · doi:10.1002/bit.10792 · Cited by 13

An original use of size exclusion-HPLC for predicting the performances of batch ultrafiltration implemented to enrich a complex protein hydrolysate in a targeted bioactive peptide

Kapel, R., Klingenberg, F., Framboisier, X., Dhulster, P., & Marc, I. (2011) · Journal of Membrane Science · doi:10.1016/j.memsci.2011.08.025 · Cited by 17

Fundamentals of Biological Separation Processes

Aires-Barros, M. R., & Azevedo, A. M. (2016) · Elsevier eBooks · doi:10.1016/b978-0-444-63668-3.00007-x · Cited by 12

High Purity of α-Lactalbumin from Binary Protein Mixture by Charged UF Membrane Far from the Isoelectric Point to Limit Fouling

Mazzei, R., Szymczak, A. M., Drioli, E., Al‐Fageeh, M. B., Aljohi, M., & Giorno, L. (2021) · Applied Sciences · doi:10.3390/app11199167 · Cited by 12

Large-Scale Production of Therapeutic Antibodies: Considerations for Optimizing Product Capture and Purification

Kemp, G., & O’Neil, P. (2004) · Springer eBooks · doi:10.1007/978-1-4419-8875-1_3 · Cited by 12

Advances in Technology and Process Development for Industrial‐Scale Monoclonal Antibody Purification

Fontes, N., & van Reis, R. (2008) · doi:10.1002/9780470444894.ch10 · Cited by 12

Grafting of oligodeoxynucleotide hairpin onto membrane surface to improve its anti-fouling performance

He, S., Liu, W., Ye, J., Wang, S., & Xu, X. (2014) · Desalination · doi:10.1016/j.desal.2014.11.027 · Cited by 11

Purification of monoclonal antibodies in a stirred cell with polyethyleneimine‐modified polyethersulfone ultrafiltration membrane

Wagner, A., Ferraria, A. M., do Rego, A. M. B., Mateus, M., & Azevedo, A. M. (2019) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.6157 · Cited by 11

High Performance Tangential Flow Filtration Using Charged Affinity Ligands

Rao, S., Ager, K., & Zydney, A. L. (2007) · Separation Science and Technology · doi:10.1080/01496390701477196 · Cited by 10

Ultrafiltration of Parvovirus

Grzenia, D. L., Wickramasinghe, S. R., & Carlson, J. O. (2007) · Separation Science and Technology · doi:10.1080/01496390701477220 · Cited by 10

Numerical study of a hybrid membrane cell with semi and fully permeable membrane sub-sections

Miranda, J. M., & Campos, J. B. L. M. (2006) · Chemical Engineering Science · doi:10.1016/j.ces.2006.11.001 · Cited by 10

Development of charged membranes for the ultrafiltration of siRNA

Banik, I., Qian, K. K., Mason, M. L., & Zydney, A. L. (2024) · Journal of Membrane Science · doi:10.1016/j.memsci.2024.123274 · Cited by 9

Bioseparations

Zydney, A. L., & van Reis, R. (2011) · Elsevier eBooks · doi:10.1016/b978-0-08-088504-9.00440-2 · Cited by 9

Production of Lactobacilli Proteinases for the Manufacture of Bioactive Peptides: Part II—Downstream Processes

Agyei, D., Potumarthi, R., & Danquah, M. K. (2013) · Wiley eBooks · doi:10.1002/9781118375082.ch11 · Cited by 9

Progress in Membrane Technology for the Enrichment of Individual Whey Proteins

Khalid, M., Ghasem, N. M., Yang, X. H., Schroën, K. G. P. H., & Nazir, A. (2025) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.5c00643 · Cited by 8

Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations

Yehl, C. J., & Zydney, A. L. (2021) · Biotechnology Progress · doi:10.1002/btpr.3221 · Cited by 8

Calibration of ultrafiltration membranes against size exclusion chromatography columns

Aimar, P., & Meireles, M. (2009) · Journal of Membrane Science · doi:10.1016/j.memsci.2009.09.018 · Cited by 8

A new membrane fractionation process based on the combination of hybrid membrane cells and differential diffusion of two solutes

Pinto, S. I. S., Rocha, T. M. G. T., Miranda, J. M., & Campos, J. B. L. M. (2009) · Desalination · doi:10.1016/j.desal.2008.02.034 · Cited by 8

Isoelectric Separation of Proteins using Charged Ultrafilter Membranes with Different Functionality under Coupled Driving Forces

Saxena, A., & Shahi, V. K. (2009) · Industrial & Engineering Chemistry Research · doi:10.1021/ie900258d · Cited by 8

Utilization of blood by-products: An in silico and experimental combined study for BSA usage

Arrutia, F., Fernandez, R. F., Menéndez, C. J., González, U., & Riera, F. A. (2017) · Scientific Reports · doi:10.1038/s41598-017-17029-2 · Cited by 8

In natura ovine whey proteins concentration by ultrafiltration combining batch and diafiltration operating modes

Pavoni, J. M. F., Leidens, N., Luchese, C. L., Baldasso, C., & Tessaro, I. C. (2020) · Journal of Food Process Engineering · doi:10.1111/jfpe.13554 · Cited by 7

Methods for purification of dairy nutraceuticals

Fee, C. J., Billakanti, J. M., & Saufi, S. M. (2010) · Elsevier eBooks · doi:10.1533/9780857090751.2.450 · Cited by 7

Bioseparations—Membrane Processes

Zydney, A. L., & van Reis, R. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.99815-5 · Cited by 7

4 High-Performance Tangential Flow Filtration for Protein Separations

Zydney, A. L., & Kuriyel, R. (2000) · Methods in biotechnology · doi:10.1007/978-1-59259-027-8_4 · Cited by 7

Tangential‐Flow Filtration for Virus Capture

Wickramasinghe, S. R. (2008) · doi:10.1002/9780470276280.ch20 · Cited by 7

Production of Lactobacilli Proteinases for the Manufacture of Bioactive Peptides: Part I—Upstream Processes

Agyei, D., Potumarthi, R., & Danquah, M. K. (2013) · Wiley eBooks · doi:10.1002/9781118375082.ch10 · Cited by 7

Pineapple

Vasiljevic, T. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817106-6.00010-1 · Cited by 6

2.2 Ultrafiltration Fundamentals and Engineering

Lutz, H. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.13635-2 · Cited by 6

Monoclonal Antibodies - Addressing the Challenges on the Manufacturing Processing of an Advanced Class of Therapeutic Agents

(2017) · Frontiers in clinical drug research. Anti-infectives/Frontiers in clinical drug research. Anti infectives · doi:10.2174/9781681084879117040007 · Cited by 6

Dairy protein fractionation and concentration using charged ultrafiltration membranes

Etzel, M. R., & Arunkumar, A. (2015) · doi:10.1002/9781118590331.ch4 · Cited by 6

Processing

Lutz, H. (2015) · Elsevier eBooks · doi:10.1016/b978-1-907568-46-6.00007-0 · Cited by 5

Electrostatic contributions to permeate flux behavior in single bovine serum albumin ultrafiltration

Wang, Y., & Rodgers, V. G. J. (2010) · Journal of Membrane Science · doi:10.1016/j.memsci.2010.10.002 · Cited by 4

Ultrafiltration

Charcosset, C. (2012) · Elsevier eBooks · doi:10.1016/b978-0-444-56334-7.00002-2 · Cited by 4

Ultrafiltration

Kilduff, J. E. (2013) · doi:10.1002/9781118522318.emst076 · Cited by 4

Analysis of the effects of electrostatic interactions on protein transport through zwitterionic ultrafiltration membranes using protein charge ladders

Hadidi, M., & Zydney, A. L. (2014) · Journal of Applied Polymer Science · doi:10.1002/app.41540 · Cited by 3

High-Throughput Process Development: II. Membrane Chromatography

Rathore, A. S., & Muthukumar, S. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-977-2_4 · Cited by 3

Nanopore Membranes for Separation and Sensing

Emilsson, G., & Dahlin, A. (2018) · Integrated analytical systems · doi:10.1007/978-3-319-64747-0_1 · Cited by 3

The effect of protein–membrane interactions on filtration behavior in forced-flow electrophoresis of protein solutions

Ahmad, A. L., & Halim, H. N. A. (2008) · Separation and Purification Technology · doi:10.1016/j.seppur.2007.12.001 · Cited by 3

Membrane-based separations for solid/liquid clarification and protein purification

Aspelund, M. T. (2010) · doi:10.31274/etd-180810-754 · Cited by 3

Charged Ultrafiltration Membrane

Zydney, A. L. (2014) · Springer eBooks · doi:10.1007/978-3-642-40872-4_129-4 · Cited by 3

Protein Ultrafiltration

van Reis, R., & Zydney, A. L. (1999) · Encyclopedia of Bioprocess Technology · doi:10.1002/0471250589.ebt184 · Cited by 3

Binary protein mixture separation and purification by a cassette having an internally staged ultrafiltration membrane stack

Feng, L., Song, Y., Straeffer, G., Bartlett, A., Carbrello, C. M., Basuray, S., & Sirkar, K. K. (2025) · Journal of Membrane Science · doi:10.1016/j.memsci.2025.124658 · Cited by 2

Solid phase extraction membranes with submicron multifunctional adsorbent particles

Tomković, T., Radovanović, F., Nastasović, A. B., Vasiljević-Radović, D. G., Marković, J., Grgur, B., & Onjia, A. E. (2014) · European Polymer Journal · doi:10.1016/j.eurpolymj.2014.12.015 · Cited by 2

Developments in Membrane Science for Downstream Processing

Crespo, J. G. (2009) · doi:10.1002/9783527626779.ch11 · Cited by 2

Concentração, purificação e fracionamento das proteinas do soro lácteo através da tecnologia de separação por membranas

Baldasso, C. (2008) · Americanae (AECID Library) · Cited by 2

Electrically enhanced membrane separation – A review

Qin, X., Geng, P., Chen, G., & Chen, G. (2026) · Separation and Purification Technology · doi:10.1016/j.seppur.2026.138904 · Cited by 1

A novel membrane based process to isolate recombinant human chemokine receptor CCR3 produced in Escherichia coli

Liu, J., Chen, C., Ge, B., Lu, J., & Cui, Z. (2012) · Journal of Membrane Science · doi:10.1016/j.memsci.2012.09.020 · Cited by 1

Novel charged ultrafiltration membranes for protein separations

Mehta, A., & Zydney, A. L. (2006) · 2006 AIChE Annual Meeting · Cited by 1

High-Throughput Process Development: II—Membrane Chromatography

Rathore, A. S., & Muthukumar, S. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0775-6_3 · Cited by 1

Permeability studies in biomimetic glycosaminoglycan-hydrogel membranes

Mattern, K. J. (2008) · DSpace@MIT (Massachusetts Institute of Technology) · Cited by 1

Comparative Study on Performance and Organic Fouling of ZrO2 Ceramic Membranes in Ultrafiltration of Synthetic Water and Wastewater Treatment Plant Effluent

Li, C. (2011) · King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) · doi:10.25781/kaust-b760q · Cited by 1

Effects of Ionic Strength and Membrane Charge on Ultrafiltration Membranes

Montoya, J. (2004) · Cited by 1

Model based process design for bioprocess optimisation: case studies on precipitation with its applications in antibody purification

Ji, Y. (2012) · UCL Discovery (University College London) · Cited by 1

Caractérisation et optimisation des phénomènes de transfert dans un double bioréacteur à membranes

Günther, J. (2009) · doi:10.70675/cc5bdb23zcf21z4a18z8a3fz417e8793913c · Cited by 1

Estudo da recuperação, concentração e purificação de biossurfactante produzido por Bacillus subtilis

Mano, M. C. R. (2008) · doi:10.47749/t/unicamp.2008.410275

Industrial Applications of Membrane Technology in Ireland: A Review

O’Dea, M., & Duffy, N. W. (2013)

Estudo da concentração do licopeno da polpa de mamão (Carica papaya L.) por ultrafiltração em escala piloto

Paes, J. (2011) · doi:10.47749/t/unicamp.2011.803184

In-situ extracellular protein isolation of yarrowia lipolytica through membrane technology: A review

Chan, D. J. C., Zulkali, M. M. D., & Ahmad, A. L. (2004)

COMPUTATIONAL STUDIES OF PROTEIN AND PARTICLE TRANSPORT IN MEMBRANE SYSTEM

Kim, M.-M. (2005)

PURIFICATION AND PRODUCTION OF PEGYLATED PROTEINS

Ruanjaikaen, K. (2013)

Active anti-fouling and defouling of membranes using electrochemical methods

Mueller, J. (2016) · The University of Queensland · doi:10.14264/uql.2017.40

Separation of Bioactive Peptides and Proteins from by-Products and Co-Products Through Membranes

Nourbakhsh, H., & Jafari, S. M. (2021) · Food bioactive ingredients · doi:10.1007/978-3-030-84643-5_6

Charged Ultrafiltration Membrane

Zydney, A. L. (2016) · Springer eBooks · doi:10.1007/978-3-662-44324-8_129

ADVANCES IN TECHNOLOGY AND PROCESS DEVELOPMENT FOR INDUSTRIAL‐SCALE MONOCLONAL ANTIBODY PURIFICATION

Fontes, N., & van Reis, R. (2017) · doi:10.1002/9781119126942.ch9

Foreword

(2007) · Separation Science and Technology · doi:10.1080/01496390701569588

Ranaspumin Protein Characterization and Applications for Both Environmental and Medical Purposes

Koerber, R. (2022) · doi:10.12794/metadc2048601

High-performance tangential flow filtration: a highly selective membrane separation process

Christy, C., Adams, G., Kuriyel, R., Bolton, G., & Seilly, A. (2002) · Desalination · doi:10.1016/s0011-9164(02)00301-6 · Cited by 39

4 High-Performance Tangential Flow Filtration for Protein Separations

Zydney, A. L., & Kuriyel, R. (2000) · Methods in biotechnology · doi:10.1007/978-1-59259-027-8_4 · Cited by 7

High performance tangential flow filtration

van Reis, R., Gadam, S. D., Frautschy, L. N., Orlando, S., Goodrich, E. M., Saksena, S., Kuriyel, R., Simpson, C. M., Pearl, S., & Zydney, A. L. (1997) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19971005)56:1 · Cited by 185

Application of high‐performance tangential flow filtration (HPTFF) to the purification of a human pharmaceutical antibody fragment expressed in Escherichia coli

Lebreton, B., Brown, A., & van Reis, R. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21842 · Cited by 62

Selective protein purification via tangential flow filtration – Exploiting protein-protein complexes to enable size-based separations

Pires, I. S., & Palmer, A. F. (2020) · Journal of Membrane Science · doi:10.1016/j.memsci.2020.118712 · Cited by 24