Cited by 38Protease and Inhibitor MechanismsReproductive biology and impacts on aquatic speciesZebrafish Biomedical Research Applications

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Sex- and tissue-specific expression of aspartic proteinases in Danio rerio (zebrafish)

Abstract

Full-length zebrafish cDNAs encoding two aspartic proteinases were cloned and sequenced. One of the two cDNAs was a 1708 bp product with an open reading frame of 398 amino acid residues corresponding to a cathepsin D. The other was a 1383 bp product encoding a polypeptide chain of 416 amino acids homologous to nothepsin, an aspartic proteinase first identified by us in the liver of Antarctic Notothenioidei. Gene expression assessed by RT-PCR and northern blot hybridization of RNA from different tissues showed that the expression was tissue- and sex-specific. Whereas the cathepsin D gene was expressed in all the tissues examined independently of the sex, the nothepsin gene was expressed exclusively in female livers.

Overview

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Registry

Crossref · 10.1016/s0378-1119(00)00469-8

Cites

30 of 30 references verified0 citation errors · 0 not checkable

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Citations

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Metadata

Authors
Riggio, M., Scudiero, R., Filosa, S., & Parisi, E.
Affiliations
  • University of Naples Federico II (IT)
  • Institute of Protein Biochemistry (IT)
Corresponding author
Parisi, Elio
Venue
Gene
Publisher
Elsevier BV
Published
2000-12
Volume
260
Issue
1-2
Pages
67-75
ISSN
0378-1119 (ISSN-L)
OpenAlex
W1989531790
Cited by
38
References
30
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2022: 2 · 2019: 1 · 2018: 3 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 4
FWCI
0.68
Type
journal article
Language
en
Open access
closed
Locations
  • Gene (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
aspartic protease, cathepsin D, zebrafish, sex-specific gene expression, tissue-specific gene expression, liver, cDNA cloning, gene expression
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cathepsin D, Female, Male, Molecular Sequence Data, Phylogeny, RNA, Sex Factors, Tissue Distribution, Zebrafish, Blotting, Northern, Gene Expression Regulation, Enzymologic, Aspartic Acid Endopeptidases, Sequence Analysis, DNA, DNA, Complementary

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