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Cited by 568

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Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression

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Edwards, Robert A, Keller, Linda H, Schifferli, Dieter M·1998·Gene

Abstract

A series of vectors has been developed to provide improved positive and negative selection for allelic exchange. Based on homologous regions of DNA ranging in size from less than 200 bp to over 1 kb, we have successfully used these new plasmids to introduce or remove markers in chromosomal or plasmid DNA. Wild type fimbria genes were replaced both in Salmonella enteritidis (sefA, agfA and fimC) and Escherichia coli (fasA and fasH). Regulation of 987P fimbriation could be identified after replacement of fasA and fasH with allelic reporter fusions. The expression of fasA but not fasH is dependent upon the osmolarity of the growth medium in an HNS-dependent manner, but unlike some other fimbrial systems expression is not dependent on the exogenous iron concentration.

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Metadata

Authors
Edwards, R. A., Keller, L. H., & Schifferli, D. M.
Venue
Gene
Publisher
Elsevier BV
Published
1998-01
Volume
207
Issue
2
Pages
149-157
ISSN
0378-1119 (ISSN-L)
OpenAlex
W2052164279
Cited by
568
References
0
FWCI
2.73
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. https://app.cochat.ai/papers/10.1016/s0378-1119(97)00619-7
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Edwards, R. A., Keller, L. H., & Schifferli, D. M. (1998). Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. Gene. https://doi.org/10.1016/s0378-1119(97)00619-7

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