CoChat
Sign Up
Cited by 83Escherichia coli research studiesMilk Quality and Mastitis in Dairy CowsMicrobial infections and disease research

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Virulence factors of Escherichia coli isolated from bovine clinical mastitis

Kaipainen, T., Pohjanvirta, T., Shpigel, N.Y., Shwimmer, A., Pyörälä, S., Pelkonen, S.·2002·Veterinary Microbiology
DOI 10.1016/s0378-1135(01)00483-7
Open this paper in CoChat — free account

Abstract

Escherichia coli isolates from bovine mastitis were examined for a selection of virulence factors. The strains originated from Finland and Israel, which have differences in the proportion of mastitis caused by E. coli, clinical pictures of coliform mastitis, environmental conditions and herd management. The genes of nine virulence factors were detected by polymerase chain reaction. Presence of K1 and K5 capsules was assessed by use of specific bacteriophages. Serum resistance was tested by a turbidimetric assay. Out of 160 Finnish isolates, 37% had traT, 14% cnf2, 8% cnf1, 11% aer, 9% f17, 8% sfa, 7% pap, 1% afa8D and 1% afa8E. Out of 113 Israeli isolates, 41% had traT, 4% aer, 3% cnf2, 1% cnf1, 1% sfa and 1% f17. Some of the genes were distributed among two major pathotype groups, with either f17 family or sfa, pap and cnf1 as major determinants. Genes for F17a, CS31A, Afa7D and Afa7E were not detected. Altogether 49% of Finnish and 42% of Israeli isolates had at least one virulence gene, but genes other than traT were present in only 24% of Finnish and 5% of Israeli isolates. Serum resistance was more common among Finnish (94/160) than Israeli isolates (19/113). K1 and K5 capsules were not detected.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0378-1135(01)00483-7

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Resistance to the bactericidal activity of serum is a virulence factor common to many coliform bacteria causing clinical mastitis, but is not a prerequisite for pathogenicity.

    “Resistance to the bactericidal activity of serum is a virulence factor common to many coliform bacteria causing clinical mastitis, but is not a prerequisite for pathogenicity [14, 26, 36, 52].”

    From Coliform mastitis (2003) · cites this paper as [36]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 28

  1. Barrow, 1989, The virulence characteristics of strains of Escherichia coli isolated from cases of bovine mastitis in England and Wales, Vet. Microbiol., 20, 35, 10.1016/0378-1135(89)90005-9 · DOI
  2. Bertin, 1996, Rapid and specific detection of F17-related pilin and adhesion genes in diarrhoeic and septicaemic Escherichia coli strains by multiplex PCR, J. Clin. Microbiol., 34, 2921, 10.1128/JCM.34.12.2921-2928.1996 · DOI
  3. Bertin, 1998, Association of genes encoding P fimbriae, CS31A antigen and EAST 1 toxin among CNF1-producing Escherichia coli strains from cattle with septicaemia and diarrhoea, FEMS Microbiol. Lett., 162, 235, 10.1111/j.1574-6968.1998.tb13004.x · DOI
  4. Burns, 1996, CNF producing Escherichia coli isolated from cattle in Northern Ireland, Vet. Microbiol., 49, 235, 10.1016/0378-1135(95)00196-4 · DOI
  5. Carroll, 1977, Bactericidal activity of standard bovine serum against coliform bacteria isolated from udders and the environment of dairy cows, Am. J. Vet. Res., 38, 2019
  6. Eberhart, 1984, Coliform mastitis, Vet. Clin. North. Am., 6, 287
  7. Hogan, 1990, Haemagglutination and hemolysis by Escherichia coli isolated from bovine intramammary infections, J. Dairy Sci., 73, 3126, 10.3168/jds.S0022-0302(90)79001-7 · DOI
  8. Lalioui, 1999, Molecular cloning and characterization of the afa-7 and afa-8 gene clusters encoding afimbrial adhesions in Escherichia coli strains associated with diarrhoea or septicaemia in calves, Infect. Immun., 67, 5048, 10.1128/IAI.67.10.5048-5059.1999 · DOI
  9. Lam, T.J.G.M., 1996. Dynamics of bovine mastitis: a field study in low somatic cell count herds. Faculteit Diergeneeskunde, Universiteit Utrecht, Utrecht, pp. 147–171.
  10. Le Bouguenec, 1999, AFA and F17 adhesions produced by pathogenic Escherichia coli strains in domestic animals, Vet. Res., 30, 317
  11. Lipman, 1995, Isolation and identification of fimbriae and toxin production by Escherichia coli strains from cows with clinical mastitis, Vet. Microbiol., 47, 1, 10.1016/0378-1135(95)00108-M · DOI · Paper page
  12. Mainil, 1997, Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family, Can. J. Vet. Res., 61, 193
  13. Mainil, 1999, Comparison of necrotoxigenic Escherichia coli isolates from farm animals and from humans, Vet. Microbiol., 70, 123, 10.1016/S0378-1135(99)00134-0 · DOI
  14. Mäkelä, P.H., 1985. The use of bacteriophages and bacteriocins in the study of bacterial cell surface structures. In: Korhonen, T.K., Dawes, E.A., Mäkelä, P.H. (Eds.), Enterobacterial Surface Antigenes: Methods for Molecular Characterisation. Elsevier, Amsterdam, pp. 155–178.
  15. Nemeth, 1991, Serum resistance and the traT gene in bovine mastitis-causing Escherichia coli, Vet. Microbiol., 28, 343, 10.1016/0378-1135(91)90069-R · DOI
  16. Nemeth, 1994, In vitro comparison of bovine mastitis and faecal Escherichia coli isolates, Vet. Microbiol., 40, 231, 10.1016/0378-1135(94)90112-0 · DOI
  17. Olling, S., 1977. Sensitivity of gram-negative bacilli to the serum bactericidal activity: a marker of the host–parasite relationship in acute and persisting infections. Scand. J. Infect. Dis. (Suppl.) 10, 1–40. · DOI
  18. Pelkonen, 1987, A rapid turbidimetric assay for the study of serum sensitivity of Escherichia coli, FEMS Microbiol., 42, 53, 10.1111/j.1574-6968.1987.tb02298.x · DOI
  19. Pfaff-McDonough, 2000, Complement resistance-related traits among Escherichia coli isolates from apparently healthy birds and birds with colibacillosis, Avian Dis., 44, 23, 10.2307/1592504 · DOI
  20. Pohl, 1995, F17 positive Escherichia coli, Vet. Rec., 137, 623
  21. Pohl, 1993, Escherichia coli producing CNF1 and CNF2 cytotoxins in animals with different disorders, Vet. Res., 24, 311
  22. Pramoonjago, 1992, Role of TraT protein, an anticomplementary protein produced in Escherichia coli by R100 factor, in serum resistance, J. Immunol., 148, 827, 10.4049/jimmunol.148.3.827 · DOI
  23. Pyörälä, 1998, Efficacy of parenteral administration of three antimicrobial agents in treatment of clinical mastitis in lactating cows: 487 cases (1989–1995), J. Am. Vet. Med. Assoc., 212, 407, 10.2460/javma.1998.212.03.407 · DOI
  24. Sanchez-Carlo, 1984, Virulence factors of Escherichia coli isolated from cows with acute mastitis, Am. J. Vet. Res., 45, 1775
  25. Sandholm, M., Pyörälä, S., 1995. Coliform mastitis, endotoxin mastitis–endotoxin shock. In: Sandholm, M., Honkanen-Buzalski, T., Kaartinen, L., Pyörälä, S. (Eds.), The Bovine Udder and Mastitis. Gummerus, Jyväskylä, pp. 149–160.
  26. Shpigel, 1998, Clinical, bacteriological and epidemiological aspects of clinical mastitis in Israeli dairy herds, Prev. Vet. Med., 35, 1, 10.1016/S0167-5877(98)00052-X · DOI
  27. Valente, 1988, Studies on virulence factors of Escherichia coli isolated from cows with acute mastitis, Arch. Vet. Ital., 39, 254
  28. Yamamoto, 1995, Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction, FEMS Immunol. Med. Microbiol., 12, 85, 10.1111/j.1574-695X.1995.tb00179.x · DOI

Cited by

Sorted by citations

Metadata

Authors
Kaipainen, T., Pohjanvirta, T., Shpigel, N. Y., Shwimmer, A., Pyörälä, S., & Pelkonen, S.
Affiliations
  • University of Helsinki (FI)
  • Hebrew University of Jerusalem (IL)
Corresponding author
Kaipainen, T.
ORCID
Venue
Veterinary Microbiology
Publisher
Elsevier BV
Published
2002-02
Volume
85
Issue
1
Pages
37-46
ISSN
0378-1135 (ISSN-L)
DOI
10.1016/s0378-1135(01)00483-7
OpenAlex
W2078391941
Cited by
83
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 6 · 2021: 2 · 2020: 6 · 2019: 6 · 2018: 2 · 2017: 7 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 3
FWCI
1.88
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Valio
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Milk Quality and Mastitis in Dairy Cows
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Escherichia coli, bovine mastitis, virulence factors, coliform mastitis, traT, cytotoxic necrotizing factor 1, sFas, PAP, serum resistance, K1 capsule
MeSH terms
Pancreatitis-Associated Proteins, Animals, Bacterial Outer Membrane Proteins, Base Sequence, Blood Bactericidal Activity, Cattle, Escherichia coli, Finland, Genes, Bacterial, Israel, Mastitis, Bovine, Nephelometry and Turbidimetry, Virulence, Polymerase Chain Reaction, Bacterial Capsules, DNA Primers, Escherichia coli Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review