Circulating heart-reactive antibodies in patients with myocarditis or cardiomyopathy
Open accessAbstract
Heart-reactive antibodies are commonly observed in patients with myocarditis or cardiomyopathy. Such antibodies may be important in the pathogenesis of these disorders, yet the specific antigens recognized have not been studied systematically. This report characterizes circulating heart autoantibodies from patients with myocarditis (n = 17) or idiopathic cardiomyopathy (n = 71) and from healthy volunteers (n = 15). Indirect immunofluorescence demonstrated that high titer (greater than or equal to 1:20) immunoglobulin G (IgG) antibody activity occurred in 59% of the myocarditis samples, 20% of the cardiomyopathy samples and none of the normal samples. All samples were tested by Western immunoblotting for IgG activity against a normal human heart extract. The number of antigens recognized by each sample was enumerated and the molecular weight of each antigen estimated; the prevalence of reactivity against antigens in selected molecular weight classes was determined. There was no difference in the mean number of heart antigens recognized by serum from each group. For most weight classes, prevalence either did not differ significantly among the various groups or subgroups or was greatest among samples from healthy volunteers. Prevalence of reactivity with 190 to 199 kilodalton (kd) antigens was greatest (p less than 0.05) among low titer serum samples from patients with myocarditis. High titer cardiomyopathy serum differed from normal serum by an increased (p less than 0.05) prevalence of antibodies to 40 to 49 and 100 to 109 kd antigens. These results suggest that western immunostaining may ultimately contribute substantively to identifying patients with myocarditis or cardiomyopathy.
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Elevated levels of antimyosin autoantibodies are present in the serum of myocarditis patients and murine models, as well as in other cardiovascular disease conditions.
“an elevated level of antimyosin autoantibodies in the serum of both myocarditis patients and murine models, as well as in other cardiovascular disease conditions”
From Autoimmune Myocarditis, Old Dogs and New Tricks. (2024) · cites this paper as [87]
The opening report (page not indexed) finds high-titer heart-reactive antibodies in 59% of myocarditis patients, but does not establish that those antibodies target myosin. The Technical considerations section reports antibodies against cardiac myosin in murine myocarditis models, and Induction of heart-reactive antibodies discusses antimyosin antibodies in other cardiac conditions. Thus the murine and other-condition portions are supported, but the claim of elevated antimyosin antibodies in the patients studied is not. (LLM, conf=0.88)
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References 40 · Cited by 191
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- Authors
- Neumann, D. A., Lynne Burek, C., Baughman, K. L., Rose, N. R., & Herskowitz, A.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Neumann, David A.
- Venue
- Journal of the American College of Cardiology
- Publisher
- Elsevier BV
- Published
- 1990-10
- Volume
- 16
- Issue
- 4
- Pages
- 839-846
- ISSN
- 0735-1097 (ISSN-L)
- OpenAlex
- W2015543995
- Cited by
- 191
- References
- 40
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 5 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 5
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of the American College of Cardiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl034744-04)
- National Heart, Lung, and Blood Institute (HL34744)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Health Sciences › Medicine › Neurology › Autoimmune Neurological Disorders and Treatments
- Keywords
- myocarditis, idiopathic cardiomyopathy, serum autoantibodies, IgG, autoantibody prevalence, autoantibody titers, disease diagnosis
- MeSH terms
- Adolescent, Adult, Aged, Antibodies, Child, Female, Fluorescent Antibody Technique, Heart Diseases, Humans, Immunoglobulin G, Male, Middle Aged, Molecular Weight, Myocarditis, Blotting, Western
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