Postsynaptically Silent Synapses in Single Neuron Cultures
Open accessAbstract
We have used the synapses that isolated hippocampal cells in culture form onto themselves (autapses) to determine if some synapses lack functional AMPA receptors (AMPARs). A comparison of the synaptic variability of the AMPAR- and NMDAR-mediated evoked responses, as well as of miniature synaptic responses, indicates that a population of events exists that only contains an NMDAR component. Spillover of glutamate from adjacent synapses cannot explain these results because in single cell cultures all synaptic events mediated by AMPARs should be detected. Immunocytochemical analysis of these cultures clearly reveals a population of synapses with puncta for NR1 (NMDAR) but not for GluR1 (AMPAR). These results provide strong anatomical and physiological evidence for the existence of postsynaptically silent synapses.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The functional mapping approach was able to detect the clustering of AMPA receptors in the postsynaptic membrane.
“Thus, our functional mapping approach was able to detect the clustering of AMPA receptors in the postsynaptic membrane23, 32, 33.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [32]
Thin spines and filopodia may be postsynaptic components of silent synapses.
“In addition, our study suggests that thin spines and filopodia may be postsynaptic components of silent synapses28, 32, 34, 44.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [32]
Cited by
Sorted by citations
Long-Term Potentiation--A Decade of Progress?
Malenka, R. C., & Nicoll, A. R. A. (1999) · Science · doi:10.1126/science.285.5435.1870 · Cited by 2,729 · see citing works
Self-citationMatsuzaki, M., Ellis-Davies, G. C. R., Nemoto, T., Miyashita, Y., Iino, M., & Kasai, H. (2001) · Nature Neuroscience · doi:10.1038/nn736 · Cited by 1,563 · see citing works
AMPARs and Synaptic Plasticity: The Last 25 Years
Huganir, R. L., & Nicoll, R. A. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.025 · Cited by 968 · see citing works
Self-citationA meta-analysis of D-cycloserine and the facilitation of fear extinction and exposure therapy
Norberg, M. M., Krystal, J. H., & Tolin, D. F. (2008) · Biological Psychiatry · doi:10.1016/j.biopsych.2008.01.012 · Cited by 510 · see citing works
Molecular aspects of glutamate dysregulation: implications for schizophrenia and its treatment
Konradi, C., & Heckers, S. (2003) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(02)00328-5 · Cited by 336 · see citing works
NMDA Receptor Content of Synapses in Stratum Radiatum of the Hippocampal CA1 Area
Racca, C., Stephenson, F. A., Streit, P., Roberts, J. D. B., & Somogyi, P. (2000) · The Journal of Neuroscience · doi:10.1523/jneurosci.20-07-02512.2000 · Cited by 304 · see citing works
Activity-Dependent Regulation of Synaptic AMPA Receptor Composition and Abundance by β3 Integrins
Cingolani, L. A., Thalhammer, A., Yu, L. M. Y., Catalano, M., Ramos, T., Colicos, M. A., & Goda, Y. (2008) · Neuron · doi:10.1016/j.neuron.2008.04.011 · Cited by 223 · see citing works
Vanderklish, P. W., Krushel, L. A., Holst, B. H., Gally, J. A., Crossin, K. L., & Edelman, G. M. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.040565597 · Cited by 163 · see citing works
Cysteine String Protein-α Prevents Activity-Dependent Degeneration in GABAergic Synapses
García-Junco-Clemente, P., Cantero, G., Gómez-Sánchez, L., Linares-Clemente, P., Martínez-López, J. A., Luján, R., & Fernández-Chacón, R. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.0924-10.2010 · Cited by 79 · see citing works
Klug, J. R., Mathur, B. N., Kash, T. L., Wang, H.-D., Matthews, R. T., Robison, A. J., Anderson, M. E., Deutch, A. Y., Lovinger, D. M., Colbran, R. J., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045323 · Cited by 49 · see citing works
- Authors
- Gomperts, S. N., Rao, A., Craig, A. M., Malenka, R. C., & Nicoll, R. A.
- Affiliations
- University of California, San Francisco (US)
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Nicoll, Roger A
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 21
- Issue
- 6
- Pages
- 1443-1451
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2115316299
- Cited by
- 142
- References
- 0
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 1 · 2015: 1 · 2014: 4 · 2013: 4 · 2012: 4
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of California, San Francisco
- National Institute of General Medical Sciences (T32GM007618, GM07618)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- autapses, AMPA receptors, NMDA receptor, hippocampal neurons, glutamate spillover, NR1 subunit, GluR1 subunit, synaptic variability
- MeSH terms
- Animals, Animals, Newborn, Cells, Cultured, Evoked Potentials, Hippocampus, Neurons, Quinoxalines, Synapses, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Receptors, AMPA, Patch-Clamp Techniques, Excitatory Amino Acid Antagonists, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
