Analysis of clock proteins in mouse SCN demonstrates phylogenetic divergence of the circadian clockwork and resetting mechanisms
Open accessAbstract
The circadian clock in the suprachiasmatic nuclei (SCN) is comprised of a cell-autonomous, autoregulatory transcriptional/translational feedback loop. Its molecular components include three period and two cryptochrome genes. We describe circadian patterns of expression of mPER2 and mPER3 in the mouse SCN that are synchronous to those for mPER1, mCRY1, and mCRY2. Coimmunoprecipitation experiments demonstrate in vivo associations of the SCN mPER proteins with each other and with the mCRY proteins, and of mCRY proteins with mTIM, but no mPER/mTIM interactions. Examination of the effects of weak and strong resetting light pulses on SCN clock proteins highlights a central role for mPER1 in photic entrainment, with no acute light effects on either the mCRY or mTIM proteins. These clock protein interactions and photic responses in mice are divergent from those described in Drosophila.
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mPER1 and mPER2 have been shown to interact in vitro and in vivo.
“As mPER1 and mPER2 have been shown to interact in vitro and in vivo (Field et al., 2000; Kume et al., 1999)”
From Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock. (2001) · cites this paper as Field et al., 2000
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References 41 · Cited by 343
- Authors
- Field, M. D., Maywood, E. S., O'Brien, J. A., Weaver, D. R., Reppert, S. M., & Hastings, M. H.
- Affiliations
- University of Cambridge (GB)
- Harvard University (US)
- Corresponding author
- Hastings, Michael H
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2000-02
- Volume
- 25
- Issue
- 2
- Pages
- 437-447
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2082351155
- Cited by
- 343
- References
- 41
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 4 · 2021: 12 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 11 · 2016: 3 · 2015: 8 · 2014: 15 · 2013: 12 · 2012: 7
- FWCI
- 15.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (R37 HD14427)
- Medical Research Council
- Biotechnology and Biological Sciences Research Council (8/S09882)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Keywords
- circadian clock, suprachiasmatic nucleus, clock proteins, Per1, Per2, Per3, CRY1, CRY2, TIM, light entrainment, transcription-translation feedback loop, phylogenetic divergence, Drosophila comparison, mice
- MeSH terms
- Animals, Circadian Rhythm, Drosophila, Eye Proteins, Flavoproteins, Male, Mice, Inbred Strains, Nuclear Proteins, Photic Stimulation, Phylogeny, RNA, Messenger, Suprachiasmatic Nucleus, Transcription Factors, Genetic Variation, Gene Expression, In Situ Hybridization, Photoreceptor Cells, Invertebrate, Cell Cycle Proteins, Drosophila Proteins, Receptors, G-Protein-Coupled, Mice, Cryptochromes, Period Circadian Proteins
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