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Cited by 131Cellular Mechanics and InteractionsProtein Kinase Regulation and GTPase SignalingMicrotubule and mitosis dynamics

Actin dynamics: Assembly and disassembly of actin networks

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Wear, Martin A, Schafer, Dorothy A, Cooper, John A·2000·Current Biology
DOI 10.1016/s0960-9822(00)00845-9
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Abstract

Assembly of branched actin filament networks at the leading edge of migrating cells requires stimulation of the Arp2/3 complex by WASp proteins, in concert with the WASp activators Cdc42, PIP(2) and profilin. Network disassembly and debranching appears to be linked to actin-bound ATP hydrolysis as filaments age.

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  • Several other proteins known to signal via actin-regulatory pathways, including VCP/neurofibromin, NESH/Abi-3 and Cofilin, drive similar effects on spine structure when either up- or downregulated.

    “As we observe an increase in immature spines upon CYFIP1 overexpression or haploinsufficiency in vitro, it is interesting to note that several other proteins known to signal via actin-regulatory pathways have been shown to drive similar effects on spine structure when either up- or downregulated, including VCP/neurofibromin, NESH/Abi-3 and Cofilin.47,48,57,64, 65, 66, 67”

    From The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines (2014) · cites this paper as [47]

    The paper’s “Future directions” section describes ADF/cofilin as acting through actin-filament severing, supporting its connection to actin regulation. It does not report effects of cofilin up- or downregulation on dendritic spines, or address VCP/neurofibromin or NESH/Abi-3. No page number is available in our copy. (LLM, conf=0.84)

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Research works printed but not linked: 37

  1. [1]1. Mitchison, TJ ∙ Cramer, LP Actin-based cell motility and cell locomotion Cell. 1996; 84:371-379 Full Text Full Text (PDF) Scopus (1327) PubMed Google Scholar
  2. [2]2. Borisy, GG ∙ Svitkina, TM Actin machinery: pushing the envelope Curr Opin Cell Biol. 2000; 12:104-112 Crossref Scopus (406) PubMed Google Scholar
  3. [3]3. Blanchoin, L ∙ Pollard, TD ∙ Mullins, RD Interactions of actophorin (ADF/cofilin), Arp2/3 complex, capping protein and profilin in the remodeling and disassembly of branched actin filament networks Curr Biol. 2000; 10:1273-1282 Full Text Full Text (PDF) Scopus (226) PubMed Google Scholar
  4. [4]4. Pollard, TD ∙ Blanchoin, L ∙ Mullins, RD Molecular mechanisms controlling actin filament dynamics in nonmuscle cells Annu Rev Biophys Biomol Struct. 2000; 29:545-576 Crossref Scopus (1223) PubMed Google Scholar
  5. [5]5. Schafer, DA ∙ Schroer, TA Actin-related proteins Annu Rev Cell Dev Biol. 1999; 15:341-363 Crossref Scopus (103) PubMed Google Scholar
  6. [6]6. Mullins, RD ∙ Pollard, TD Structure and function of the Arp2/3 complex Curr Opin Struct Biol. 1999; 9:244-249 Crossref Scopus (95) PubMed Google Scholar
  7. [7]7. Svitkina, TM ∙ Borisy, GG Arp2/3 complex and actin depolymerizing factor cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia J Cell Biol. 1999; 145:1009-1026 Crossref Scopus (941) PubMed Google Scholar
  8. [8]8. Schafer, DA ∙ Welch, MD ∙ Machesky, LM ... Visualization and molecular analysis of actin assembly in living cells J Cell Biol. 1998; 143:1919-1930 Crossref Scopus (154) PubMed Google Scholar
  9. [9]9. Mullins, RD ∙ Heuser, JA ∙ Pollard, TD The interaction of Arp2/3 complex with actin-nucleation, high affinity pointed end capping, and formation of branching networks of filaments Proc Natl Acad Sci USA. 1998; 95:6181-6186 Crossref Scopus (1086) PubMed Google Scholar
  10. [10]10. Loisel, TP ∙ Boujemaa, R ∙ Pantaloni, D ... Reconstitution of actin-based motility of Listeria and Shigella using pure proteins Nature. 1999; 401:613-616 Crossref Scopus (824) PubMed Google Scholar
  11. [11]11. Welch, MD ∙ Rosenblatt, J ∙ Skoble, J ... Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation Science. 1998; 281:105-108 Crossref Scopus (428) PubMed Google Scholar
  12. [12]12. Higgs, HN ∙ Pollard, TD Regulation of actin polymerization by Arp2/3 complex and WASp/Scar proteins J Biol Chem. 1999; 274:32531-32534 Full Text Full Text (PDF) Scopus (210) PubMed Google Scholar
  13. [13]13. Hall, A Rho GTPases and the actin cytoskeleton Science. 1998; 279:509-514 Crossref Scopus (5315) PubMed Google Scholar
  14. [14]14. Mullins, RD How WASP-family proteins and the Arp2/3 complex convert intracellular signals into cytoskeletal structures Curr Opin Cell Biol. 2000; 12:91-96 Crossref Scopus (141) PubMed Google Scholar
  15. [15]15. Miki, H ∙ Suetsugu, S ∙ Takenawa, T WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac EMBO J. 1998; 17:6932-6941 Crossref Scopus (582) PubMed Google Scholar
  16. [16]16. Bear, JE ∙ Rawls, JF ∙ Saxe, CL 3rd SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development J Cell Biol. 1998; 142:1325-1335 Crossref Scopus (233) PubMed Google Scholar
  17. [17]17. Zigmond, SH How WASP regulates actin polymerization J Cell Biol. 2000; 150:117-120 Crossref PubMed Google Scholar
  18. [18]18. Rohatgi, R ∙ Ma, L ∙ Miki, H ... The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly Cell. 1999; 97:221-231 Full Text Full Text (PDF) Scopus (1105) PubMed Google Scholar
  19. [19]19. Machesky, LM ∙ Mullins, RD ∙ Higgs, HN ... Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex Proc Natl Acad Sci USA. 1999; 96:3739-3744 Crossref Scopus (635) PubMed Google Scholar
  20. [20]20. Miki, H ∙ Sasaki, T ∙ Takai, Y ... Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP Nature. 1998; 391:93-96 Crossref Scopus (573) PubMed Google Scholar
  21. [21]21. Miki, H ∙ Miura, K ∙ Takenawa, T N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases EMBO J. 1996; 15:5326-5335 Crossref Scopus (564) PubMed Google Scholar
  22. [22]22. Prehoda, KE ∙ Lee, DJ ∙ Lim, WA Structure of the enabled/VASP homology 1 domain-peptide complex: a key component in the spatial control of actin assembly Cell. 1999; 97:471-480 Full Text Full Text (PDF) Scopus (207) PubMed Google Scholar
  23. [23]23. Suetsugu, S ∙ Miki, H ∙ Takenawa, T The essential role of profilin in the assembly of actin for microspike formation EMBO J. 1998; 17:6516-6526 Crossref Scopus (196) PubMed Google Scholar
  24. [24]24. Carlier, MF ∙ Nioche, P ∙ Broutin-L'Hermite, I ... GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin-related protein (ARP2/3) complex J Biol Chem. 2000; 275:21946-21952 Full Text Full Text (PDF) Scopus (184) PubMed Google Scholar
  25. [25]25. Kim, AS ∙ Kakalis, LT ∙ Abdul-Manan, N ... Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein Nature. 2000; 404:151-158 Crossref Scopus (641) PubMed Google Scholar
  26. [26]26. Higgs, HN ∙ Pollard, TD Activation by cdc42 and PIP(2) of wiskott-aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex J Cell Biol. 2000; 150:1311-1320 Crossref Scopus (424) PubMed Google Scholar
  27. [27]27. Rohatgi, R ∙ Ho, H ∙ Kirschner, MW Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4,5- bisphosphate J Cell Biol. 2000; 150:1299-1310 Crossref Scopus (511) PubMed Google Scholar
  28. [28]28. Castellano, F ∙ Montcourrier, P ∙ Guillemot, JC ... Inducible recruitment of Cdc42 or WASP to a cell-surface receptor triggers actin polymerization and filopodium formation Curr Biol. 1999; 9:351-360 Full Text Full Text (PDF) Scopus (133) PubMed Google Scholar
  29. [29]29. Yang, C ∙ Huang, M ∙ DeBiasio, J ... Profilin enhances Cdc42-induced nucleation of actin polymerization J Cell Biol. 2000; 150:1001-1012 Crossref Scopus (103) PubMed Google Scholar
  30. [30]30. Higgs, HN ∙ Blanchoin, L ∙ Pollard, TD Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization Biochemistry. 1999; 38:15212-15222 Crossref Scopus (248) PubMed Google Scholar
  31. [31]31. Blanchoin, L ∙ Amann, KJ ∙ Higgs, HN ... Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins Nature. 2000; 404:1007-1011 Crossref Scopus (452) PubMed Google Scholar
  32. [32]32. Pantaloni, D ∙ Boujemaa, R ∙ Didry, D ... The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins Nat Cell Biol. 2000; 2:385-391 Crossref Scopus (208) PubMed Google Scholar
  33. [33]33. Bamburg, JR Proteins of the ADF/cofilin family: essential regulators of actin dynamics Annu Rev Cell Dev Biol. 1999; 15:185-230 Crossref Scopus (867) PubMed Google Scholar
  34. [34]34. Blanchoin, L ∙ Pollard, TD Mechanism of interaction of Acanthamoeba actophorin (ADF/Cofilin) with actin filaments J Biol Chem. 1999; 274:15538-15546 Full Text Full Text (PDF) Scopus (257) PubMed Google Scholar
  35. [35]35. McGough, A ∙ Pope, B ∙ Chiu, W ... Cofilin changes the twist of F-actin: implications for actin filament dynamics and cellular function J Cell Biol. 1997; 138:771-781 Crossref Scopus (608) PubMed Google Scholar
  36. [36]36. Bear, JE ∙ Loureiro, JJ ∙ Libova, I ... Negative regulation of fibroblast motility by Ena/VASP proteins Cell. 2000; 101:717-728 Full Text Full Text (PDF) Scopus (385) PubMed Google Scholar
  37. [37]37. Chan, AY ∙ Bailly, M ∙ Zebda, N ... Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion J Cell Biol. 2000; 148:531-542 Crossref Scopus (212) PubMed Google Scholar

Cited by

Sorted by citations

Authors
Wear, M. A., Schafer, D. A., & Cooper, J. A.
Affiliations
Washington University in St. Louis (US)
Corresponding author
Cooper, John A
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2000-12
Volume
10
Issue
24
Pages
R891-R895
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/s0960-9822(00)00845-9
OpenAlex
W2098136597
Cited by
131
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 3 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 6 · 2018: 6 · 2017: 3 · 2016: 4 · 2015: 1 · 2014: 7 · 2013: 1 · 2012: 4
FWCI
1.00
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (GM-38542)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
actin dynamics, actin filament assembly, branched actin networks, Arp2/3 complex, WASP protein, Cdc42, phosphatidylinositol 4,5-bisphosphate, profilin, cell migration, leading edge
MeSH terms
Actins, Animals, Humans, Microfilament Proteins, Models, Biological, Polymers, Proteins, Protein Structure, Tertiary, Wiskott-Aldrich Syndrome Protein

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