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Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi
Open accessAbstract
RNA interference (RNAi) is a broadly used reverse genetics method in C. elegans. Unfortunately, RNAi does not inhibit all genes. We show that loss of function of a putative RNA-directed RNA polymerase (RdRP) of C. elegans, RRF-3, results in a substantial enhancement of sensitivity to RNAi in diverse tissues. This is particularly striking in the nervous system; neurons that are generally refractory to RNAi in a wild-type genetic background can respond effectively to interference in an rrf-3 mutant background. These data provide the first indication of physiological negative modulation of the RNAi response and implicate an RdRP-related factor in this effect. The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens.
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Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
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Metadata
- Authors
- Simmer, F., Tijsterman, M., Parrish, S., Koushika, S. P., Nonet, M. L., Fire, A., Ahringer, J., & Plasterk, R. H. A.
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2002-08
- Volume
- 12
- Issue
- 15
- Pages
- 1317-1319
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2101276421
- Cited by
- 576
- References
- 1
- FWCI
- 13.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Simmer, F., Tijsterman, M., Parrish, S., Koushika, S. P., Nonet, M. L., Fire, A., Ahringer, J., & Plasterk, R. H. A. (2002). Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi. Current Biology. https://doi.org/10.1016/s0960-9822(02)01041-2
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