CoChat
Sign Up
Cited by 189Developmental Biology and Gene RegulationRNA Research and SplicingMicrotubule and mitosis dynamics

The DOI matches its Crossref record.

The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila

Open access
Micklem, David R., Dasgupta, Ramanuj, Elliott, Heather, Gergely, Fanni, Davidson, Catherine, Brand, Andrea, González-Reyes, Acaimo, St Johnston, Daniel·1997·Current Biology
DOI 10.1016/s0960-9822(06)00218-1
Open this paper in CoChat — free accountOpen PDF

Abstract

Background Drosophila axis formation requires a series of inductive interactions between the oocyte and the somatic follicle cells. Early in oogenesis, Gurken protein, a member of the transforming growth factor alpha family, is produced by the oocyte to induce the adiacent follicle cells to adopt a posterior cell fate. These cells subsequently send an unidentified signal back to the oocyte to induce the formation of a polarised microtubule array that defines the anterior-posterior axis. The polarised microtubules also direct the movement of the nucleus and gurken mRNA from the posterior to the anterior of the oocyte, where Gurken signals a second time to induce the dorsal follicle cells, thereby polarising the dorsal-ventral axis. Results In addition to its previously described role in the localisation of oskar mRNA, the mago nashi gene is required in the germ line for the transduction of the polarising signal from the posterior follicle cells. Using a new in vivo marker for microtubules, we show that mago nashi mutant oocytes develop a symmetric microtubule cytoskeleton that leads to the transient localisation of bicoid mRNA to both poles. Furthermore, the oocyte nucleus often fails to migrate to the anterior, causing the second Gurken signal to be sent in the same direction as the first. This results in a novel phenotype in which the anterior of the egg is ventralised and the posterior dorsalised, demonstrating that the migration of the oocyte nucleus determines the relativ…

Overview

Integrity signals

Registry

Crossref · 10.1016/s0960-9822(06)00218-1

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 47

  1. Grünert, 1996, RNA localization and the development of asymmetry during Drosophila oogenesis, Curr Opin Genet Dev, 6, 395, 10.1016/S0959-437X(96)80059-1 · DOI
  2. González-Reyes, 1994, Role of oocyte position in establishment of anterior–posterior polarity in Drosophila, Science, 266, 639, 10.1126/science.7939717 · DOI
  3. Neuman-Silberberg, 1993, The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGF alpha-like protein, Cell, 75, 165, 10.1016/S0092-8674(05)80093-5 · DOI
  4. González-Reyes, 1995, Polarization of both major body axes in Drosophila by gurken-torpedo signalling, Nature, 375, 654, 10.1038/375654a0 · DOI
  5. Roth, 1995, cornichon and the EGF receptor signaling process are necessary for both anterior–posterior and dorsal–ventral pattern formation in Drosophila, Cell, 81, 967, 10.1016/0092-8674(95)90016-0 · DOI
  6. Ruohola, 1991, Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila, Cell, 66, 433, 10.1016/0092-8674(81)90008-8 · DOI
  7. Ruohola-Baker, 1994, The role of gene cassettes in axis formation during Drosophila oogenesis, Trends Genet, 10, 89, 10.1016/0168-9525(94)90231-3 · DOI
  8. Theurkauf, 1992, Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport, Development, 115, 923, 10.1242/dev.115.4.923 · DOI
  9. Theurkauf, 1993, A central role for microtubules in the differentiation of Drosophila oocytes, Development, 118, 1169, 10.1242/dev.118.4.1169 · DOI
  10. Lane, 1994, RNA localization along the anteroposterior axis of the Drosophila oocyte requires PKA-mediated signal transduction to direct normal microtubule organization, Genes Dev, 8, 2986, 10.1101/gad.8.24.2986 · DOI
  11. Giniger, 1993, Tracing neurons with a kinesin–galactosidase fusion protein, Roux's Arch Dev Biol, 202, 112, 10.1007/BF00636536 · DOI
  12. Clark, 1994, Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte, Curr Biol, 4, 289, 10.1016/S0960-9822(00)00068-3 · DOI
  13. Clark, 1997, Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle, Development, 124, 461, 10.1242/dev.124.2.461 · DOI
  14. Pokrywka, 1991, Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis, Development, 113, 55, 10.1242/dev.113.1.55 · DOI
  15. Pokrywka, 1995, Microtubules are a general component of messenger RNA localization systems in Drosophila oocytes, Dev Biol, 167, 363, 10.1006/dbio.1995.1030 · DOI
  16. St Johnston, 1995, The intracellular localisation of messenger RNAs, Cell, 81, 161, 10.1016/0092-8674(95)90324-0 · DOI
  17. Micklem, 1995, mRNA localisation during development, Dev Biol, 172, 377, 10.1006/dbio.1995.8048 · DOI
  18. St Johnston, 1992, The origin of pattern and polarity in the Drosophila Embryo, Cell, 68, 201, 10.1016/0092-8674(92)90466-P · DOI
  19. Ephrussi, 1992, Induction of germ cell formation by oskar, Nature, 358, 387, 10.1038/358387a0 · DOI
  20. Koch, 1983, Multiple effects of colchicine on oogenesis in Drosophila: induced sterility and switch of potential oocyte to nurse cell developmental pathway, Cell Tissue Res, 228, 21, 10.1007/BF00206261 · DOI
  21. Schüpbach, 1987, Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster, Cell, 49, 699, 10.1016/0092-8674(87)90546-0 · DOI · Paper page
  22. Boswell, 1991, Mutations in a newly identified Drosophila melanogaster gene, mago nashi, disrupt germ cell formation and result in the formation of mirror-image symmetrical double abdomen embryos, Development, 113, 373, 10.1242/dev.113.1.373 · DOI
  23. Newmark, 1994, The mago nashi locus encodes an essential product required for germ plasm assembly in Drosophila, Development, 120, 1303, 10.1242/dev.120.5.1303 · DOI
  24. Fasano, 1988, Monitoring positional information during oogenesis in adult Drosophila, Development, 104, 245, 10.1242/dev.104.2.245 · DOI
  25. Brand, 1995, GFP in Drosophila, Trends Genet, 11, 324, 10.1016/S0168-9525(00)89091-5 · DOI
  26. Theurkauf, 1992, Behavior of structurally divergent alpha-tubulin isotypes during Drosophila embryogenesis: evidence for post-translational regulation of isotype abundance, Dev Biol, 154, 205, 10.1016/0012-1606(92)90060-T · DOI
  27. Chou, 1996, The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster, Genetics, 144, 1673, 10.1093/genetics/144.4.1673 · DOI
  28. Kim-Ha, 1991, oskar mRNA is localized to the posterior pole of the Drosophila oocyte, Cell, 66, 23, 10.1016/0092-8674(91)90136-M · DOI
  29. Ephrussi, 1991, oskar organizes the germ plasm and directs localization of the posterior determinant nanos, Cell, 66, 37, 10.1016/0092-8674(91)90137-N · DOI
  30. Goode, 1996, The neurogenic genes egghead and brainiac define a novel signaling pathway essential for epithelial morphogenesis during Drosophila oogenesis, Development, 122, 3863, 10.1242/dev.122.12.3863 · DOI
  31. Goode, 1996, brainiac encodes a novel putative secreted protein that cooperates with grk TGFα to produce the follicular epithelium, Dev Biol, 178, 35, 10.1006/dbio.1996.0196 · DOI
  32. Altschul, 1990, Basic local alignment search tool, J Mol Biol, 215, 403, 10.1016/S0022-2836(05)80360-2 · DOI · Paper page
  33. Grossniklaus, 1989, P-element-mediated enhancer detection applied to the study of oogenesis in Drosophila, Development, 107, 189, 10.1242/dev.107.2.189 · DOI
  34. O’Donnel, 1989, A cytogenetic analysis of the Punch-tudor region of chromosome 2R in Drosophila melanogaster, Genetics, 121, 273, 10.1093/genetics/121.2.273 · DOI
  35. Bier, 1989, Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector, Genes Dev, 3, 1273, 10.1101/gad.3.9.1273 · DOI
  36. Heim, 1994, Wavelength mutations and posttranslational auto oxidation of green fluorescent protein, Proc Natl Acad Sci USA, 91, 12501, 10.1073/pnas.91.26.12501 · DOI
  37. Heim, 1995, Improved green fluorescence, Nature, 373, 663, 10.1038/373663b0 · DOI
  38. Callahan, 1994, Tau–galactosidase, an axon-targeted fusion protein, Proc Natl Acad Sci USA, 91, 5972, 10.1073/pnas.91.13.5972 · DOI
  39. Butner, 1991, Tau protein binds to microtubules through a flexible array of distributed weak sites, J Cell Biol, 115, 717, 10.1083/jcb.115.3.717 · DOI · Paper page
  40. Thummel, 1988, Vectors for Drosophila P-element-mediated transformation and tissue culture transfection, Gene, 74, 445, 10.1016/0378-1119(88)90177-1 · DOI
  41. Theurkauf, 1986, Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins, Proc Natl Acad Sci USA, 83, 8477, 10.1073/pnas.83.22.8477 · DOI
  42. Matthews, 1989, Developmental distribution of RNA and protein products of the Drosophila α-Tubulin gene family, Dev Biol, 132, 45, 10.1016/0012-1606(89)90203-0 · DOI
  43. St Johnston, 1991, Staufen, a gene required to localize maternal RNAs in the Drosophila egg, Cell, 66, 51, 10.1016/0092-8674(91)90138-O · DOI
  44. Tautz, 1989, A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback, Chromosoma, 98, 81, 10.1007/BF00291041 · DOI
  45. Brown, 1988, Functional cDNA libraries from Drosophila embryos, J Mol Biol, 203, 425, 10.1016/0022-2836(88)90010-1 · DOI
  46. Xu, 1993, Analysis of genetic mosaics, Development, 117, 1223, 10.1242/dev.117.4.1223 · DOI
  47. Smith, 1996, BCM Search Launcher – an integrated interface to molecular biology data base search and analysis services available on the World Wide Web, Genome Res, 6, 454, 10.1101/gr.6.5.454 · DOI

Cited by

Sorted by citations

See all 189

Metadata

Authors
Micklem, D. R., Dasgupta, R., Elliott, H., Gergely, F., Davidson, C., Brand, A., González-Reyes, A., & St Johnston, D.
Affiliations
University of Cambridge (GB)
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
1997-07
Volume
7
Issue
7
Pages
468-478
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/s0960-9822(06)00218-1
OpenAlex
W1970284326
Cited by
189
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2022: 4 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 10 · 2015: 4 · 2014: 10 · 2013: 4 · 2012: 6
FWCI
2.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
oocyte polarity, Drosophila oogenesis, axis formation, follicle cells, microtubule cytoskeleton, anterior-posterior axis, dorsal-ventral axis
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cell Nucleus, DNA, Drosophila, Female, Humans, Microtubules, Molecular Sequence Data, Mutation, Nuclear Proteins, Oocytes, Transforming Growth Factors, Trans-Activators, Transforming Growth Factor alpha, RNA-Binding Proteins, Cell Polarity, Genes, Insect, Sequence Homology, Amino Acid, Homeodomain Proteins, Insect Proteins, Body Patterning, Drosophila Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review