Age dependent increase of elastase type protease activity in mouse skin
Abstract
The effect of chronological aging and photoaging (UV-radiation) on elastase-type enzyme activity of hairless mouse skin was studied. Aging resulted in the increase of elastase type endopeptidase activity extractable from mouse skins. Both chronic UVA and UVB radiation resulted in a significant increase of elastase type activity. PBS extracted only small part of the elastase activity, UV-A produced an increase of about 90-120% according to the type of irradiation (xenon or UV-A SUN) and UV-B produced a 72% increase. Extraction by Triton X-100 suggested that most of the activity is bound to cells and fibrous structures. EDTA inhibited 80-90% of the elastase activity in chronologically aged skin extracts and also the activity induced by UVA radiation suggesting that metallo-elastase(s) are involved. About 30% of the UVB induced activity could only be inhibited by EDTA and about 50% by PMSF suggesting that irradiation by UVB increased more serine endopeptidase activity but also MMP-activity. Chronic UVA radiation produced an increase of skin elastase activity equivalent to that observed after 24 months of aging in non-irradiated animals (approximately 100 weeks) corresponding to approximately 90% of total life span of these mice. The total increase produced by UVB was less, but the strong increase of a serine elastase, presumably from PMN-s, appear to produce a much more pronounced biological activity as shown by the presence of fibronectin degradation products in skin extracts.…
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PDRN inhibits elastase and MMP-1, preserving elastin integrity, improving skin elasticity, and reducing wrinkle depth.
“Concurrently, it inhibits elastase and matrix metalloproteinase-1 (MMP-1), which are enzymes responsible for ECM degradation, preserving elastin integrity, improving skin elasticity, and reducing wrinkle depth [91,92,93].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [92]
In dermal fibroblasts, PDRN enhances metabolic activity and promotes the synthesis of collagen types I and III through activation of the ERK pathway.
“In dermal fibroblasts, PDRN enhances metabolic activity and promotes the synthesis of essential ECM components—particularly collagen types I and III—through activation of the extracellular signal-regulated kinase (ERK) pathway [52,89,90,91,92,93,94].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [92]
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References 29 · Cited by 90
- Authors
- Labat-Robert, J., Fourtanier, A., Boyer-Lafargue, B., & Robert, L.
- Affiliations
- L'Oréal (France) (FR)
- Hôtel-Dieu de Paris (FR)
- Corresponding author
- Robert, L
- Venue
- Journal of Photochemistry and Photobiology B: Biology
- Publisher
- Elsevier BV
- Published
- 2000-09
- Volume
- 57
- Issue
- 2-3
- Pages
- 113-118
- ISSN
- 1011-1344 (ISSN-L)
- OpenAlex
- W194825863
- Cited by
- 90
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 4 · 2019: 6 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Photochemistry and Photobiology B Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Keywords
- elastase, skin aging, photoaging, ultraviolet radiation, UVA, UV-B radiation, metalloproteinases, neutrophils, fibronectin degradation, elastase activity, hairless mouse skin, extracellular matrix
- MeSH terms
- Aging, Animals, Female, Fibronectins, Pancreatic Elastase, Skin, Ultraviolet Rays, Mice
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