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Activation Domain–Mediated Targeting of the SWI/SNF Complex to Promoters Stimulates Transcription from Nucleosome Arrays
Open accessAbstract
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.
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“Because VP48 is known to directly interact with chromatin-remodeling cofactors21–23”
From Single-molecule states link transcription factor binding to gene expression (2024) · cites this paper as [23]
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Metadata
- Authors
- Neely, K. E., Hassan, A. H., Wallberg, A. E., Steger, D. J., Cairns, B. R., Wright, A. P. H., & Workman, J. L.
- Affiliations
- Pennsylvania State University (US)
- Södertörn University (SE)
- University of Utah (US)
- Corresponding author
- Workman, Jerry L
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 1999-10
- Volume
- 4
- Issue
- 4
- Pages
- 649-655
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2119383396
- Cited by
- 245
- References
- 53
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 8 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 7 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 8 · 2013: 2 · 2012: 5
- FWCI
- 9.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- SWI/SNF complex, chromatin remodeling, transactivation domain, nucleosome arrays, transcription initiation, nucleosome structure, SAGA complex, histone acetyltransferase, protein-protein interaction, Swi5, HAP4, GCN4, VP16
- MeSH terms
- Chromatin, DNA-Binding Proteins, Fungal Proteins, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Kinases, Recombinant Proteins, Transcription Factors, Yeasts, Transcriptional Activation, Gene Expression Regulation, Fungal, Herpes Simplex Virus Protein Vmw65, Cell Cycle Proteins, CCAAT-Binding Factor, Saccharomyces cerevisiae Proteins
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