Engineering cellular microenvironments to improve cell-based drug testing
Abstract
Recent progress in the biology of cell adhesion is enabling cell culture models to better reproduce in vivo functions. Cues from adhesion to extracellular matrix and neighboring cells are important regulators of cell behaviors. The recent adaptation of semiconductor tools to spatially organize cells and their adhesions has enhanced our ability to engineer cell functions ex vivo. By using these tools to create more in vivo-like cultures, cell-based drug discovery and target validation could be improved. This review explores the biological advances made by these microfabrication tools and discusses how they could enable high-throughput cell-based assays.
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2D cell culture tests sometimes provide misleading and nonpredictive data for in vivo responses.
“As a result, 2D cell culture tests sometimes provide misleading and nonpredictive data for in vivo responses. 1-3”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [3]
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References 71 · Cited by 355
- Authors
- Bhadriraju, K., & Chen, C. S.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Chen, Christopher S
- Venue
- Drug Discovery Today
- Publisher
- Elsevier BV
- Published
- 2002-05
- Volume
- 7
- Issue
- 11
- Pages
- 612-620
- ISSN
- 1359-6446 (ISSN-L)
- OpenAlex
- W2140474512
- Cited by
- 355
- References
- 71
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 11 · 2023: 15 · 2022: 21 · 2021: 19 · 2020: 19 · 2019: 19 · 2018: 25 · 2017: 22 · 2016: 23 · 2015: 13 · 2014: 11 · 2013: 20 · 2012: 8
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Drug Discovery Today (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office of Naval Research
- Defense Advanced Research Projects Agency
- National Institute of General Medical Sciences (GM60692)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- cell microenvironment, cell adhesion, extracellular matrix, microfabrication, target validation
- MeSH terms
- Animals, Cell Differentiation, Cell Division, Cells, Cultured, Drug Evaluation, Preclinical, Endothelium, Vascular, Humans, Phenotype, Semiconductors, Drug Design, Hepatocytes
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