The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media
Open accessAbstract
Abstract With increasing use of proteins in many different applications, ranging from phramaceuticals to biosensors and biomaterials, there has emerged a need for protein structural characterisation in diverse environments. In many cases it is not sufficient to just have the three-dimensional structure of a protein in H 2 O or in the crystalline state. Often information on the structural properties of a protein is required in the presence of organic solvents, detergent micelles, phospholipid membranes and so on. Fourier transform infrared spectroscopy (FTIR) has been identified as one of the few techniques that can be applied for structural characterisation of proteins in such environments. Here we discuss how this technique is being used to obtain information on protein structure and stability in both aqueous and non-aqueous media. Examples are drawn from our studies of water soluble proteins and membrane proteins.
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Cites
Protein secondary structures in water from second-derivative amide I infrared spectra
Dong, A., Huang, P., & Caughey, W. S. (1990) · Biochemistry · doi:10.1021/bi00465a022 · Cited by 1,333
Cited by
ZiCo: A Peptide Designed to Switch Folded State upon Binding Zinc
Cerasoli, E., Sharpe, B. K., & Woolfson, D. N. (2005) · Journal of the American Chemical Society · doi:10.1021/ja0543604 · Cited by 104
Ding, Y., Ma, H., Wang, K., Azam, S. M. R., Wang, Y., Zhou, J., & Qu, W. (2021) · LWT · doi:10.1016/j.lwt.2021.111320 · Cited by 81
Metadata
- Authors
- Haris, P. I., & Severcan, F.
- Venue
- Journal of Molecular Catalysis B: Enzymatic
- Publisher
- Elsevier BV
- Published
- 1999-09
- Volume
- 7
- Issue
- 1-4
- Pages
- 207-221
- ISSN
- 1381-1177 (ISSN-L)
- OpenAlex
- W2002257633
- Cited by
- 533
- References
- 1
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Haris, P. I., & Severcan, F. (1999). FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media. Journal of Molecular Catalysis B: Enzymatic. https://doi.org/10.1016/s1381-1177(99)00030-2
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