The DOI matches its Crossref record.
Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability
Abstract
A novel analytical method for analysis of microbial quinones in activated sludge sample was developed with improved reliability compared to the conventional method. The operating conditions for the extraction of quinones from activated sludge sample with a methanol-chloroform mixture and hexane were optimized. A solid phase extraction method using the Sep-Pak Plus Silica, a small column packed with silica gel, was employed for the purification and separation of quinones instead of column chromatography and thin layer column chromatography used in the conventional method. The analytical operation was simplified and the analytical time was shortened by a half or two-thirds of that required for the conventional method. The recoveries of quinones were markedly increased from 30-60% with the conventional method to as high as 90-101% with the improved method, demonstrating that the reliability of the analytical results of the improved method is significantly higher than that of the conventional method. The quinone content value obtained using the improved method was twice as high as that obtained using the conventional method for the same activated sludge sample, but the quinone profiles expressed as the mole fraction of each quinone type were the same for each method.
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In the citing paper
“The subsequent extraction with solvent from the Sep-Pak Plus silica has led to a recovery of 90-100% for bacterial quinones, compared to the conventional recovery of about 30 to 60% obtained with column chromatography and TLC [144].”
From Natural Quinone Dyes: A Review on Structure, Extraction Techniques, Analysis and Application Potential (2021) · cites this paper as [144]
In the citing paper
“The main advantage of this method is the ability to separate the matrix from the target compound, automation ability, and ability to concentrate the final product [144].”
From Natural Quinone Dyes: A Review on Structure, Extraction Techniques, Analysis and Application Potential (2021) · cites this paper as [144]
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No references could be resolved yet.
Research works printed but not linked: 9
- Hedrick, 1986, Microbial respiratory quinones in the environment, J. Microbiol. Method, 5, 243, 10.1016/0167-7012(86)90049-7 · DOI
- Hiraishi, 1988, Respiratory quinone profiles as tools for identifying different bacterial populations in activated sludge, J. Gen. Appl. Microbiol., 34, 39, 10.2323/jgam.34.39 · DOI
- Fujie, 1994, Ecological studies of aerobic submerged biofilter on the basis of respiratory quinone profiles, Wat. Sci. Tech., 29, 373, 10.2166/wst.1994.0364 · DOI
- Hu, 1996, Studies of microbial acclimation to hard chemicals on the basis of respiratory quinone profiles and kinetic analyses, Wat. Sci. Tech., 33, 249, 10.2166/wst.1996.0557 · DOI
- Hu, 1997, Respiratory quinone profile as a tool for refractory chemical biodegradation study, Wat. Sci. Tech., 35, 103, 10.1016/S0273-1223(97)00156-X · DOI
- Collins, 1981, Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications, Microbiological Rev., 45, 316, 10.1128/MR.45.2.316-354.1981 · DOI
- Hu, 1994, Effect of temperature on the reaction rate of bacteria inhabiting the aerobic microbial film for wastewater treatment, J. Ferment. Bioeng., 78, 100, 10.1016/0922-338X(94)90187-2 · DOI
- Fujie, 1998, Analysis of respiratory quinone profile in soil for characterization of microbiota, Soil Sci. Plant Nutr., 44, 393, 10.1080/00380768.1998.10414461 · DOI
- Hiraishi, 1991, Numerical analysis of lipoquinone patterns in monitoring bacterial community dynamics in wastewater treatment, J. Gen. Appl. Microbiol., 37, 57, 10.2323/jgam.37.57 · DOI
Cited by
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Dulo, B., Phan, K., Githaiga, J., Raes, K., & De Meester, S. (2021) · Waste and Biomass Valorization · doi:10.1007/s12649-021-01443-9 · Cited by 181
Metadata
- Authors
- Hu, H.-Y., Fujie, K., & Urano, K.
- Affiliations
- Toyohashi University of Technology (JP)
- Yokohama National University (JP)
- Corresponding author
- Hu, Hong-Ying
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 1999-01
- Volume
- 87
- Issue
- 3
- Pages
- 378-382
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W1977334924
- Cited by
- 70
- References
- 0
- Citations by year
- 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Physical Sciences › Environmental Science › Waste Management and Disposal › Water Quality Monitoring and Analysis
- Keywords
- activated sludge, solid-phase extraction, silica gel column, extraction optimization
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Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability. https://app.cochat.ai/papers/10.1016/s1389-1723(99)80049-8
- The paper
- Hu, H.-Y., Fujie, K., & Urano, K. (1999). Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability. Journal of Bioscience and Bioengineering. https://doi.org/10.1016/s1389-1723(99)80049-8
