A Viral Phospholipase A2 Is Required for Parvovirus Infectivity
Open accessAbstract
Sequence analysis revealed phospholipase A2 (PLA2) motifs in capsid proteins of parvoviruses. Although PLA2 activity is not known to exist in viruses, putative PLA2s from divergent parvoviruses, human B19, porcine parvovirus, and insect GmDNV (densovirus from Galleria mellonella), can emulate catalytic properties of secreted PLA2. Mutations of critical amino acids strongly reduce both PLA2 activity and, proportionally, viral infectivity, but cell surface attachment, entry, and endocytosis by PLA2-deficient virions are not affected. PLA2 activity is critical for efficient transfer of the viral genome from late endosomes/lysosomes to the nucleus to initiate replication. These findings offer the prospect of developing PLA2 inhibitors as a new class of antiviral drugs against parvovirus infections and associated diseases.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A phospholipase domain essential for viral infectivity has been identified in the unique N terminus of Vp1.
“A phospholipase domain, essential for viral infectivity, has been identified in the unique N terminus of Vp1 (19, 53).”
From Packaging Capacity of Adeno-Associated Virus Serotypes: Impact of Larger Genomes on Infectivity and Postentry Steps (2005) · cites this paper as [53]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 59 · Cited by 518
Research works printed but not linked: 18
- [1]1. Balsinde, J. ∙ Balboa, M.A. ∙ Insel, P.A. ... Regulation and inhibition of phospholipase A2 Annu. Rev. Pharmacol. Toxicol. 1999; 39:175-189 Crossref Scopus (543) PubMed Google Scholar
- [2]2. Bergeron, J. ∙ Menezes, J. ∙ Tijssen, P. Genomic organization and mapping of transcription and translation products of the NADL-2 strain of porcine parvovirus Virology. 1993; 197:86-98 Crossref Scopus (65) Google Scholar
- [3]3. Bergeron, J. ∙ Hebert, B. ∙ Tijssen, P. Genome organization of the Kresse strain of porcine parvovirusidentification of the allotropic determinant and comparison with those of NADL-2 and field isolates J. Virol. 1996; 70:2508-2515 Crossref PubMed Google Scholar
- [4]4. Berns, K.I. Parvoviridaethe viruses and their replication Third Edition, B.N. ∙ Fields, D.M. ∙ Knipe ... (Editors) Fundamental Virology Lippincott-Raven, Philadelphia, PA, 1996 1017–1041.pp Google Scholar
- [5]5. Brown, K.E. Haematological consequences of parvovirus B19 infection Baillieres Best Pract. Res. Clin. Haematol. 2000; 13:245-259 Crossref Scopus (85) PubMed Google Scholar
- [6]6. Brown, K.E. ∙ Anderson, S.M. ∙ Young, N.S. Erythrocyte P antigencellular receptor for B19 parvovirus Science. 1993; 262:114-117 Crossref Scopus (752) PubMed Google Scholar
- [7]7. Chapman, M.S. ∙ Rossmann, M.G. Structure, sequence and function correlations among parvoviruses Virology. 1993; 194:491-508 Crossref Scopus (186) PubMed Google Scholar
- [8]8. Cotmore, S.F. ∙ D'Abramo, Jr., M.A. ∙ Ticknor, C.M. ... Controlled conformational transitions in the MVM virion expose the VP1 N-terminus and viral genome without particle disassembly Virology. 1999; 254:169-181 Crossref Scopus (119) PubMed Google Scholar
- [9]9. Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes Trends Biochem. Sci. 1997; 22:1-2 Full Text (PDF) Scopus (767) PubMed Google Scholar
- [10]10. Dessen, A. Phospholipase A(2) enzymesstructural diversity in lipid messenger metabolism Structure. 2000; 8:R15-R22 Full Text Full Text (PDF) Scopus (28) PubMed Google Scholar
- [11]11. Dijkstra, B.W. ∙ Drenth, J. ∙ Kalk, K.H. Active site and catalytic mechanism of phospholipase A2 Nature. 1981; 289:604-606 Crossref Scopus (239) PubMed Google Scholar
- [13]13. Duan, D. ∙ Li, Q. ∙ Kao, A.W. ... Dynamin is required for recombinant adeno-associated virus type 2 infection J. Virol. 1999; 73:10371-10376 Crossref PubMed Google Scholar
- [14]14. Elsbach, P. ∙ Weiss, J. Utilization of labeled Escherichia coli as phospholipase substrate Methods Enzymol. 1991; 197:24-31 Crossref Scopus (60) PubMed Google Scholar
- [15]15. Foto, F. ∙ Saag, K.G. ∙ Scharosch, L.L. ... Parvovirus B19-specific DNA in bone marrow from B19 arthropathy patientsevidence for B19 virus persistence J. Infect. Dis. 1993; 167:744-748 Crossref Scopus (105) PubMed Google Scholar
- [16]16. Gardiner, E.M. ∙ Tattersall, P. Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated J. Virol. 1988; 62:1713-1722 Crossref Scopus (63) Google Scholar
- [17]17. Gelb, M.H. ∙ Cho, W. ∙ Wilton, D.C. Interfacial binding of secreted phospholipase A2more than electrostatics and a major role for tryptophan Curr. Opin. Struct. Biol. 1999; 9:428-432 Crossref Scopus (121) PubMed Google Scholar
- [18]18. Geuze, H.J. ∙ Stoorvogel, W. ∙ Strous, G.J. ... Sorting of mannose-6-phosphate receptors and lysosomal membrane proteins in endocytic vesicles J. Cell Biol. 1988; 107:2491-2501 Crossref Scopus (210) PubMed Google Scholar
- [19]19. Griffiths, G. ∙ Hoflack, B. ∙ Simons, K. ... The mannose-6-phosphate receptor and the biogenesis of lysosomes Cell. 1988; 52:329-341
- Authors
- Zádori, Z., Szelei, J., Lacoste, M.-C., Li, Y., Gariépy, S., Raymond, P., Allaire, M., Nabi, I. R., & Tijssen, P.
- Affiliations
- Institut National de la Recherche Scientifique (CA)
- Université de Montréal (CA)
- Corresponding author
- Tijssen, Peter
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 1
- Issue
- 2
- Pages
- 291-302
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2001738143
- Cited by
- 518
- References
- 59
- Citations by year
- 2026: 11 · 2025: 21 · 2024: 30 · 2023: 20 · 2022: 20 · 2021: 33 · 2020: 23 · 2019: 22 · 2018: 20 · 2017: 15 · 2016: 19 · 2015: 18 · 2014: 22 · 2013: 30 · 2012: 21
- FWCI
- 12.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Hungarian Scientific Research Fund
- Canadian Institutes of Health Research
- Magyar Tudományos Akadémia
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- parvovirus, phospholipase A2, capsid protein, viral infectivity, parvovirus B19, porcine parvovirus, densovirus, antiviral drug targets, PLA2 inhibitors
- MeSH terms
- Amino Acid Sequence, Calcium, Capsid, Cell Nucleus, Chromatography, Thin Layer, DNA, Genetic Vectors, Lysosomes, Microscopy, Fluorescence, Models, Biological, Models, Molecular, Molecular Sequence Data, Mutation, Phospholipases A, Endosomes, Thioredoxins, Transfection, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, In Situ Hybridization, Protein Structure, Tertiary, Parvovirus, Amino Acid Motifs, Phospholipases A2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
