The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Self- and Actin-Templated Assembly of Mammalian Septins
Open accessAbstract
Septins are polymerizing GTPases required for cytokinesis and cortical organization. The principles by which they are targeted to, and assemble at, specific cell regions are unknown. We show that septins in mammalian cells switch between a linear organization along actin bundles and cytoplasmic rings, approximately 0.6 microm in diameter. A recombinant septin complex self-assembles into rings resembling those in cells. Linear organization along actin bundles was reconstituted by adding an adaptor protein, anillin. Perturbation of septin organization in cells by expression of a septin-interacting fragment of anillin or by septin depletion via siRNA causes loss of actin bundles. We conclude that septins alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that septins help organize these bundles.
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“Septins are responsible for the organization of F-actin within the CCF and bundles F-actin [53] as found in the submembranous cellular cortex which are necessary to drive successful cellular division.”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [53]
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Self-citation
Research works printed but not linked: 1
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Metadata
- Authors
- Kinoshita, M., Field, C. M., Coughlin, M. L., Straight, A. F., & Mitchison, T. J.
- Affiliations
- Harvard University (US)
- Corresponding author
- Kinoshita, Makoto
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 3
- Issue
- 6
- Pages
- 791-802
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2055376777
- Cited by
- 526
- References
- 36
- Citations by year
- 2026: 13 · 2025: 9 · 2024: 15 · 2023: 14 · 2022: 23 · 2021: 20 · 2020: 26 · 2019: 21 · 2018: 25 · 2017: 17 · 2016: 23 · 2015: 23 · 2014: 24 · 2013: 21 · 2012: 24
- FWCI
- 8.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministério da Educação e Ciência
- National Institutes of Health (GM23928)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- septins, actin bundles, anillin, septin ring, cytokinesis, cortical organization, GTPase, adaptor proteins, actin cytoskeleton organization, mammalian cells
- MeSH terms
- Animals, Binding Sites, Blood Proteins, Carrier Proteins, Cell Compartmentation, Contractile Proteins, Cytoskeletal Proteins, Eukaryotic Cells, HeLa Cells, Humans, Luminescent Proteins, Actin Cytoskeleton, Recombinant Fusion Proteins, 3T3 Cells, RNA-Binding Proteins, Protein Structure, Tertiary, GTP-Binding Proteins, GTP Phosphohydrolase Activators, GTP-Binding Protein Regulators, GTP Phosphohydrolases, rho GTP-Binding Proteins, cdc42 GTP-Binding Protein, Adaptor Proteins, Signal Transducing, Green Fluorescent Proteins, Mice, Septins
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