The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Expression of c-fos, rather than c-jun or glucocorticoid-receptor mRNA, correlates with decreased glucocorticoid response of peripheral blood mononuclear cells in asthma
Abstract
Resolution of the molecular mechanism(s) underlying glucocorticoid (GC) resistance is an important clinical problem when performing individualized GC therapy according to the GC response of peripheral cells in asthma. In order to investigate the mechanism(s) underlying the individual differences of lymphocyte GC response, we examined the relationship between lymphocyte sensitivity to GC in vitro and the expression of mRNAs for GC receptor (GR) alpha, GRbeta, c-fos and c-jun, which are reported to be implicated in the regulation of the pharmacological effects of GCs in asthma patients. Twenty-seven patients with bronchial asthma and 14 healthy subjects were included in the study. IC50s of prednisolone and methylprednisolone on blastogenesis of peripheral blood mononuclear cells (PBMCs) stimulated with concanavalin A in vitro were estimated. Transcripts for GRalpha, c-fos, c-jun and beta-actin genes in PBMCs were quantitatively determined by reverse transcription-competitive polymerase chain reaction (RT-cPCR) procedures. GRbeta mRNA expression was examined with an RT-PCR technique. A statistically significant positive correlation was observed between the IC50s for prednisolone (p <0.002) or methylprednisolone (p <0.001) and expression of c-fos mRNA in PBMCs of asthma patients (n = 27). Thus, the increased expression of c-fos mRNA correlated with the decreased responses of PBMCs to prednisolone and methylprednisolone in vitro. In contrast, the expression of GRalpha and c-jun mR…
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“cAMP and PKA signaling pathways also interact with cytokine signaling pathways, including NF-κB, MAPK and Jak-STAT pathways.114–116”
From Cytokines and Glucocorticoid Receptor Signaling (2009) · cites this paper as [114]
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“NF-kB transcription is blocked by increased PKA activity through interaction of the catalytic subunit of PKA with p65, which stops p65 transactivation114 (Fig. 2).”
From Cytokines and Glucocorticoid Receptor Signaling (2009) · cites this paper as [114]
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Update on glucocorticoid action and resistance
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Metadata
- Authors
- Takahashi, E., Onda, K., Hirano, T., Oka, K., Maruoka, N., Tsuyuguchi, M., Matsumura, Y., Niitsuma, T., & Hayashi, T.
- Affiliations
- Tokyo University of Pharmacy and Life Sciences (JP)
- Tokyo Medical University (JP)
- Corresponding author
- Onda, Kenji
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2002-09
- Volume
- 2
- Issue
- 10
- Pages
- 1419-1427
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W1996546697
- Cited by
- 20
- References
- 31
- Citations by year
- 2024: 1 · 2020: 1 · 2018: 2 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Immunopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (09672337)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Asthma and respiratory diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- glucocorticoid resistance, asthma, c-fos, c-Jun, glucocorticoid receptor alpha, glucocorticoid receptor beta, peripheral blood mononuclear cells, prednisolone, methylprednisolone, glucocorticoid response, lymphocyte proliferation, concanavalin A, gene expression
- MeSH terms
- Adult, Aged, Aged, 80 and over, Asthma, Female, Glucocorticoids, Humans, Leukocytes, Mononuclear, Male, Middle Aged, Receptors, Glucocorticoid, RNA, Messenger, Genes, jun, Genes, fos
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