Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 6

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Paracetamol treatment increases telomerase activity in rat embryonic liver cells

Bader, Augustinus, Oliver, Petters, Keller, Mario, Pavlica, Sanja·2011·Pharmacological Reports
DOI 10.1016/s1734-1140(11)70707-1
Open this paper in CoChat — free account

Abstract

Although paracetamol is known to have a damaging effect, this pharmaceutical is widely applied to pregnant and lactating women. Despite substantial progress in our understanding of its hepatotoxicity, some mechanisms, particularly of its embryonal and developmental toxicity, are still unknown. Thus, cell culture assays that investigate its toxicity are of particular interest. We assessed the effects of acute paracetamol treatment on cell viability (LDH assay, MTT assay), glutathione content (GSH assay), metabolic status (albumin and urea assays) and telomerase activity using rat embryonic liver cells (RLC-18 cells). Incubation with low (6 mmol/l) and high (15 mmol/l) concentrations of toxin for 24 h leads to 20% and 50% cytotoxicity, respectively. Paracetamol exerted its toxicity in a similar pathway (depletion of GSH stores) as in adult liver cells, producing damage at the cellular level. Interestingly, paracetamol treatment significantly enhanced telomerase activity. Mechanisms involved in paracetamol-induced inhibition of cell senescence should be further elucidated. Telomerase activity in RLC-18 cells offers unique opportunities for examining basic biologic mechanisms. Our findings should encourage further studies to investigate a link between telomerase activity and toxicity, implying a role of impaired telomerase activity in human pathology.

Overview

Integrity signals

Registry

Crossref · 10.1016/s1734-1140(11)70707-1

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 1 references

Research works printed but not linked: 19

  1. Adams, 2002, Enhanced acetaminophen hepatotoxicity in transgenic mice overexpressing BCL-2, Mol Pharmacol, 60, 907, 10.1124/mol.60.5.907 · DOI
  2. Byer, 1982, Acetaminophen overdose in the third trimester of pregnancy, Jama, 247, 3114, 10.1001/jama.1982.03320470060034 · DOI
  3. Farkas, 2005, Characterization of chemically induced hepatotoxicity in collagen sandwiches of rat hepatocytes, Toxicol Sci, 85, 927, 10.1093/toxsci/kfi145 · DOI
  4. Harley, 2005, Telomerase therapeutics for degenerative diseases, Curr Mol Med, 205, 11
  5. Kim, 1995, Specific association of human telomerase activity with immortal cells and cancer, Science, 268, 1115
  6. Kitteringham, 2000, Hepatocellular response to chemical stress in CD-1 mice: induction of early genes and γ-glutamylcysteine synthetase, Hepatology, 32, 321, 10.1053/jhep.2000.9602 · DOI
  7. Larson, 2007, Acetaminophen hepatotoxicity, Clin Liver Dis, 11, 525, 10.1016/j.cld.2007.06.006 · DOI
  8. Levy, 1975, Evidence of placental transfer of acetaminophen, Pediatrics, 55, 895, 10.1542/peds.55.6.895 · DOI
  9. Ludmir, 1986, Maternal acetaminophen overdose at 15 weeks of gestation, Obstet Gynecol, 67, 750, 10.1097/00006250-198605000-00028 · DOI
  10. Morishita, 2006, Gene expression profile in liver of differing ages of rats after single oral administration of acetaminophen, J Toxicol Sci, 31, 491, 10.2131/jts.31.491 · DOI
  11. Pavlica, 2005, Protective effects of ellagic and chlorogenic acids against oxidative stress in PC12 cells, Free Radic Res, 39, 1377, 10.1080/09670260500197660 · DOI
  12. Pavlica, 2009, Rat embryonic liver cell expansion and differentiation on NH3 plasma-grafted PEEK-WC-PU membranes, Biomaterials, 30, 6514, 10.1016/j.biomaterials.2009.08.024 · DOI
  13. Pavlica, 2011, Effect of native and NH3 plasma-functionalized membranes on the gene expression profiles of primary hepatocytes, J Tissue Eng Regen Med
  14. Rollins, 1979, Acetaminophen: potentially toxic metabolite formed by human fetal and adult liver microsomes and isolated fetal liver cells, Science, 205, 1414, 10.1126/science.38505 · DOI
  15. Rousar, 2009, Evaluation of oxidative status in acetaminophen treated rat hepatocytes in culture, Physiol Res, 58, 239, 10.33549/physiolres.931437 · DOI
  16. Shen, 2006, Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers, Chem Biol Interact, 162, 53, 10.1016/j.cbi.2006.05.005 · DOI
  17. Takaoka, 1975, A simple method for the cultivation of rat liver cells, Jpn J Exp Med, 45, 317
  18. Weeks, 1990, Acetaminophen toxicity to cultured rat embryos, Teratog Carcinog Mutagen, 10, 361, 10.1002/tcm.1770100502 · DOI
  19. Wege, 2007, Telomerase activation in liver regeneration and hepatocarcinogenesis: Dr, Jekyll or Mr. Hyde? Curr Stem Cell Res Ther, 2, 31, 10.2174/157488807779317062 · DOI

Cited by

See all 6

Metadata

Authors
Bader, A., Oliver, P., Keller, M., & Pavlica, S.
Venue
Pharmacological Reports
Publisher
Springer Science and Business Media LLC
Published
2011-11
Volume
63
Issue
6
Pages
1435-1441
ISSN
1734-1140
DOI
10.1016/s1734-1140(11)70707-1
Cited by
6
References
1
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Paracetamol treatment increases telomerase activity in rat embryonic liver cells. https://app.cochat.ai/papers/10.1016/s1734-1140(11)70707-1
The paper
Bader, A., Oliver, P., Keller, M., & Pavlica, S. (2011). Paracetamol treatment increases telomerase activity in rat embryonic liver cells. Pharmacological Reports. https://doi.org/10.1016/s1734-1140(11)70707-1