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Cited by 52Estrogen and related hormone effectsGrowth Hormone and Insulin-like Growth FactorsHypothalamic control of reproductive hormones

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Expression of estrogen receptor α and β in the epiphyseal plate of the rat

Van der Eerden, B.C.J, Gevers, E.F, Löwik, C.W.G.M, Karperien, M, Wit, J.M·2002·Bone
DOI 10.1016/s8756-3282(01)00703-7
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Abstract

In this study we examine the spatial and temporal expression of estrogen receptor (ER) alpha and beta mRNA and protein in the tibial growth plate of the rat after birth, as well as the hormonal regulation of their expression. Using in situ hybridization and immunohistochemistry, we demonstrated ER alpha and ER beta mRNA and protein in tibial growth plates from 1 to 40 weeks after birth. ER alpha and beta mRNA and protein were localized in late proliferating and early hypertrophic chondrocytes during early life (1 and 4 weeks of age), whereas the immunohistochemistry also showed staining for ER alpha and beta in the resting cells. A similar expression pattern was observed during sexual maturation (7 weeks of age) except that ER beta mRNA was also detected in early proliferating chondrocytes. After sexual maturation (from 12 up to 40 weeks of age) ER alpha and beta mRNA and protein expression was confined to late proliferating and early hypertrophic chondrocytes. Apart from a relatively higher ER alpha mRNA expression in males after sexual maturation, we did not detect differences in expression of ERs between genders. Expression of ER beta mRNA in epiphyseal plates was increased in growth-retarded hypophysectomized rats compared with controls. Administration of growth hormone (GH) did not reverse the increased ER expression to normal. These data suggest that ER alpha and beta are coexpressed in growth plates of the rat after birth and that the level of expression of ERs in thes…

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References 40 · Cited by 52

Research works printed but not linked: 36

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Metadata

Authors
Van der Eerden, B. C. J., Gevers, E. F., Löwik, C. W. G. M., Karperien, M., & Wit, J. M.
Affiliations
Leiden University Medical Center (NL)
Corresponding author
Van der Eerden, B.C.J
ORCID
Venue
Bone
Publisher
Elsevier BV
Published
2002-03
Volume
30
Issue
3
Pages
478-485
ISSN
  • 1873-2763 (ISSN-L)
  • 8756-3282
DOI
10.1016/s8756-3282(01)00703-7
OpenAlex
W1965600017
Cited by
52
References
40
Citations by year
2026: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
3.18
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Bone (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords
estrogen receptor alpha, estrogen receptor beta, growth plate, chondrocytes, chondrocyte hypertrophy, sexual maturation, growth hormone, hypophysectomy, growth retardation, growth plate closure, hormone regulation of gene expression, sex differences
MeSH terms
Animals, Female, Growth Plate, Male, Receptors, Estrogen, RNA, Messenger, Growth Hormone, Rats, Wistar, Gene Expression Regulation, Developmental, Estrogen Receptor alpha, Estrogen Receptor beta, Rats

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