The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Subfemtomole MS and MS/MS Peptide Sequence Analysis Using Nano-HPLC Micro-ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Open accessAbstract
Subfemtomole peptide sequence analysis has been achieved using microcapillary HPLC columns, with integrated nanoelectrospray emitters, coupled directly to a Fourier transform ion cyclotron resonance mass spectrometer. Accurate mass (+/-0.010 Da) peptide maps are generated from a standard six-protein digest mixture, whose principle components span a concentration dynamic range of 1000:1. Iterative searches against approximately 189000 entries in the OWL database readily identify each protein, with high sequence coverage (20-60%), from as little as 10 amol loaded on-column. In addition, a simple variable-flow HPLC apparatus provides for on-line tandem mass spectrometric analysis of tryptic peptides at the 400-amol level. MS/MS data are searched against approximately 280000 entries in a nonredundant protein database using SEQUEST. Accurate precursor and product ion mass information readily identifies primary amino acid sequences differing by asparagine vs aspartic acid (deltam = 0.98 Da) and glutamine vs lysine (deltam = 0.036 Da).
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Cited by
Mass spectrometry-based proteomics
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Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
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Self-citationPerformance Characteristics of Electron Transfer Dissociation Mass Spectrometry
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Protein identification using sequential ion/ion reactions and tandem mass spectrometry
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Self-citationMicrocolumns with self-assembled particle frits for proteomics
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Metadata
- Authors
- Martin, S. E., Shabanowitz, J., Hunt, D. F., & Marto, J. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-08-12
- Volume
- 72
- Issue
- 18
- Pages
- 4266-4274
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1963848474
- Cited by
- 276
- References
- 40
- FWCI
- 15.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Martin, S. E., Shabanowitz, J., Hunt, D. F., & Marto, J. A. (2000). Subfemtomole MS and MS/MS Peptide Sequence Analysis Using Nano-HPLC Micro-ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Analytical Chemistry. https://doi.org/10.1021/ac000497v
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