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Two-Dimensional Electrochromatography/Capillary Electrophoresis on a Microchip
Abstract
A two-dimensional separation system on a microfabricated device was demonstrated using open-channel electrochromatography as the first dimension and capillary electrophoresis as the second dimension. The first dimension was operated under isocratic conditions, and the effluent from the first dimension was repetitively injected into the second dimension every few seconds. A 25-cm separation channel with spiral geometry for open-channel electrochromatography was chemically modified with octadecylsilane and coupled to a 1.2-cm straight separation channel for capillary electrophoresis. Fluorescently labeled products from tryptic digests of beta-casein were analyzed in 13 min with this system.
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Citations
Cited by
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,102
Micro Total Analysis Systems. 2. Analytical Standard Operations and Applications
Auroux, P.-A., Iossifidis, D., Reyes, D. R., & Manz, A. (2002) · Analytical Chemistry · doi:10.1021/ac020239t · Cited by 1,532
Abgrall, P., & Gué, A.-M. (2007) · Journal of Micromechanics and Microengineering · doi:10.1088/0960-1317/17/5/r01 · Cited by 561
Microfluidic chips for clinical and forensic analysis
Verpoorte, E. (2002) · ELECTROPHORESIS · doi:10.1002/1522-2683(200203)23:5<677::aid-elps677>3.0.co;2-8 · Cited by 526
Yin, H., Killeen, K., Brennen, R., Sobek, D., Werlich, M., & van de Goor, T. (2005) · Analytical Chemistry · doi:10.1021/ac049068d · Cited by 362
Metadata
- Authors
- Gottschlich, N., Jacobson, S. C., Culbertson, C. T., & Ramsey, J. M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-04-26
- Volume
- 73
- Issue
- 11
- Pages
- 2669-2674
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1974898170
- Cited by
- 208
- References
- 0
- FWCI
- 22.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Two-Dimensional Electrochromatography/Capillary Electrophoresis on a Microchip. https://app.cochat.ai/papers/10.1021/ac001019n
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- Gottschlich, N., Jacobson, S. C., Culbertson, C. T., & Ramsey, J. M. (2001). Two-Dimensional Electrochromatography/Capillary Electrophoresis on a Microchip. Analytical Chemistry. https://doi.org/10.1021/ac001019n
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
