The DOI matches its Crossref record.
Packed Capillary Reversed-Phase Liquid Chromatography with High-Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Proteomics
Abstract
In this study, high-efficiency packed capillary reversed-phase liquid chromatography (RPLC) coupled on-line with high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometry has been investigated for the characterization of complex cellular proteolytic digests. Long capillary columns (80-cm) packed with small (3-micron) C18 bonded particles provided a total peak capacity of approximately 1000 for cellular proteolytic polypeptides when interfaced with an ESI-FTICR mass spectrometer under composition gradient conditions at a pressure of 10,000 psi. Large quantities of cellular proteolytic digests (e.g., 500 micrograms) could be loaded onto packed capillaries of 150-micron inner diameter without a significant loss of separation efficiency. Precolumns with suitable inner diameters were found useful for improving the elution reproducibility without a significant loss of separation quality. Porous particle packed capillaries were found to provide better results than those containing nonporous particles because of their higher sample capacity. Two-dimensional analyses from the combination of packed capillary RPLC with high-resolution FTICR yield a combined capacity for separations of > 1 million polypeptide components and simultaneously provided information for the identification of the separated components based upon the accurate mass tag concept previously described.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Adsorbed Proteins Influence the Biological Activity and Molecular Targeting of Nanomaterials
Dutta, D., Sundaram, S. K., Teeguarden, J. G., Riley, B. J., Fifield, L. S., Jacobs, J. M., Addleman, S. R., Kaysen, G. A., Moudgil, B. M., & Weber, T. J. (2007) · Toxicological Sciences · doi:10.1093/toxsci/kfm217 · Cited by 447
Liu, T., Qian, W.-J., Gritsenko, M. A., Camp, D. G., Monroe, M. E., Moore, R. J., & Smith, R. D. (2005) · Journal of Proteome Research · doi:10.1021/pr0502065 · Cited by 405
Self-citationZhang, Z., Wu, S., Stenoien, D. L., & Paša-Tolić, L. (2014) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-071213-020216 · Cited by 323
Self-citationIon mobility–mass spectrometry: a new paradigm for proteomics
McLean, J. A., Ruotolo, B. T., Gillig, K. J., & Russell, D. H. (2005) · International Journal of Mass Spectrometry · doi:10.1016/j.ijms.2004.10.003 · Cited by 309
Baas, T., Baskin, C. R., Diamond, D. L., García-Sastre, A., Bielefeldt-Ohmann, H., Tumpey, T. M., Thomas, M. J., Carter, V. S., Teal, T. H., Van Hoeven, N., et al. (2006) · Journal of Virology · doi:10.1128/jvi.00851-06 · Cited by 121
Self-citation
Metadata
- Authors
- Shen, Y., Zhao, R., Belov, M. E., Conrads, T. P., Anderson, G. A., Tang, K., Paša-Tolić, L., Veenstra, T. D., Lipton, M. S., Udseth, H. R., & Smith, R. D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-16
- Volume
- 73
- Issue
- 8
- Pages
- 1766-1775
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2032961810
- Cited by
- 187
- References
- 0
- FWCI
- 12.10
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Packed Capillary Reversed-Phase Liquid Chromatography with High-Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Proteomics. https://app.cochat.ai/papers/10.1021/ac0011336
- The paper
- Shen, Y., Zhao, R., Belov, M. E., Conrads, T. P., Anderson, G. A., Tang, K., Paša-Tolić, L., Veenstra, T. D., Lipton, M. S., Udseth, H. R., & Smith, R. D. (2001). Packed Capillary Reversed-Phase Liquid Chromatography with High-Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Proteomics. Analytical Chemistry. https://doi.org/10.1021/ac0011336
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
