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Temperature Measurement in Microfluidic Systems Using a Temperature-Dependent Fluorescent Dye
Abstract
A technique is described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. In addition, the method can be used to measure fluid temperatures with micrometer spatial resolution and millisecond time resolution. The efficacy of the method is demonstrated by measuring temperature distributions resulting from Joule heating in a variety of microfluidic circuits that are electrokinetically pumped. With the equipment used for these measurements, fluid temperatures ranging from room temperature to 90 degrees C were measured with a precision ranging from 0.03 to 3.5 degrees C-dependent on the amount of signal averaging done. The spatial and temporal resolutions achieved were 1 microm and 33 ms, respectively.
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Cited by
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Baffou, G., Quidant, R., & García de Abajo, F. J. (2010) · ACS Nano · doi:10.1021/nn901144d · Cited by 760
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Yang, Z., Shi, J., Xie, J., Wang, Y., Sun, J., Liu, T., Zhao, Y., Zhao, X., Wang, X., Ma, Y., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0485-1 · Cited by 744
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Baffou, G., Quidant, R., & Girard, C. (2009) · Applied Physics Letters · doi:10.1063/1.3116645 · Cited by 593
Liu, C., Zhao, J., Tian, F., Cai, L., Zhang, W., Feng, Q., Chang, J., Wan, F., Yang, Y., Dai, B., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0343-6 · Cited by 482
Metadata
- Authors
- Ross, D., Gaitan, M., & Locascio, L. E.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-07-24
- Volume
- 73
- Issue
- 17
- Pages
- 4117-4123
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2009477800
- Cited by
- 631
- References
- 0
- FWCI
- 8.01
- Type
- journal article
- Language
- en
- Open access
- closed
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