The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
DNA Sequence Detection Using Selective Fluorescence Quenching of Tagged Oligonucleotide Probes by Gold Nanoparticles
Abstract
Simple, fast, economical, and sensitive detection of specific DNA sequences is crucial to pathogen detection and biomedical research. We have designed a novel fluorescent assay for DNA hybridization based on the electrostatic properties of DNA. We exploit the ability to create conditions where single-stranded DNA adsorbs on negatively charged gold nanoparticles while double-stranded DNA does not. Dye-tagged probe sequences have their fluorescence efficiently quenched when they are mixed with gold nanoparticles unless they hybridize with components of the analyte. Subfemtomole amounts of untagged target are detected in minutes using commercially available materials. Target sequences in complex mixtures of DNA and single-base mismatches in DNA sequences are easily detected.
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Citations
Cites
Fluidity of Bound Hydration Layers
Raviv, U., & Klein, J. (2002) · Science · doi:10.1126/science.1074481 · Cited by 721
Cited by
Toxicity and Environmental Risks of Nanomaterials: Challenges and Future Needs
RAY, P. C., YU, H., & FU, P. P. (2009) · Journal of Environmental Science and Health, Part C · doi:10.1080/10590500802708267 · Cited by 936
Metadata
- Authors
- Li, H., & Rothberg, L. J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-08-14
- Volume
- 76
- Issue
- 18
- Pages
- 5414-5417
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2061755273
- Cited by
- 272
- References
- 1
- FWCI
- 4.48
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
