The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Derivatization for Stabilizing Sialic Acids in MALDI-MS
Abstract
While matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is useful in oligosaccharide analysis, the sialic acid, or N-acetylneuraminic acid (NANA), moiety of an oligosaccharide is liable to dissociation in- or postsource during mass measurement. In this study, we tried to stabilize the moiety by amidation, as in the case of peptides (Sekiya, S.; Wada, Y. Tanaka, K. Anal. Chem. 2004, 76, 5894-5902), and found 4-(4,6-dimethoxy-1,3,5-triazin-2yl)-4-methylmorpholinium chloride to be a desirable condensing agent. Amidation stabilized the glycosidic bond with NANA and suppressed its preferential cleavage by in-source decay, postsource decay, or collision-induced dissociation. In addition, the suppressed dissociation considerably improved the yield of the B/Y type ions for structural analysis by MS/MS. These results demonstrate that amidation is an effective derivatization to reinforce the structural analysis of sialylated oligosaccharides by MALDI-MS. In addition, amidation with (15)N-labeled ammonium chloride decreases the mass shift from the acid to amide form to just 0.013, reducing the complexity of mass spectral interpretation and database searching.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers.
Hillenkamp, F., Karas, M., Beavis, R. C., & Chait, B. T. (1991) · Analytical Chemistry · doi:10.1021/ac00024a716 · Cited by 1,930
Cited by
The MALDI-TOF Mass Spectrometric View of the Plasma Proteome and Peptidome
Hortin, G. L. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2006.069252 · Cited by 351
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Harvey, D. J. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.11.010 · Cited by 260
Capillary electrophoresis–mass spectrometry for direct structural identification of serum N-glycans
Snyder, C. M., Zhou, X., Karty, J. A., Fonslow, B. R., Novotny, M. V., & Jacobson, S. C. (2017) · Journal of Chromatography A · doi:10.1016/j.chroma.2017.09.009 · Cited by 58
“Table 1 Peak area ratios for N-glycans identified as co-migrating in microchip electrophoresis ( lactonization/amidation reaction As previously reported [50,51], (cid:2) (cid:2) electrophoretic mobilities to the lactonized/amidated glycans with higher electrophoretic mobilities.”
Metadata
- Authors
- Sekiya, S., Wada, Y., & Tanaka, K.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-06-29
- Volume
- 77
- Issue
- 15
- Pages
- 4962-4968
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2033253313
- Cited by
- 175
- References
- 1
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Derivatization for Stabilizing Sialic Acids in MALDI-MS. https://app.cochat.ai/papers/10.1021/ac050287o
- The paper
- Sekiya, S., Wada, Y., & Tanaka, K. (2005). Derivatization for Stabilizing Sialic Acids in MALDI-MS. Analytical Chemistry. https://doi.org/10.1021/ac050287o
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
