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High-Resolution Length Sorting and Purification of DNA-Wrapped Carbon Nanotubes by Size-Exclusion Chromatography
Abstract
We report a size-exclusion chromatography (SEC) process to purify DNA-wrapped carbon nanotubes (DNA-CNT) and to sort them into fractions of uniform length. A type of silica-based column resin was identified that shows minimum adsorption of DNA-CNT. Three such columns in series with pore sizes of 2000, 1000, and 300 A were found to separate DNA-CNT into fractions of very narrow length distribution, as measured directly by atomic force microscopy. The average length decreases monotonically from > 500 nm in the early fractions to < 100 nm in the late fractions, with length variation < or = 10% in each of the measured fractions. Using UV-vis-NIR spectroscopy, we showed that SEC is very effective in removing graphitic impurities that contribute to the spectral baseline and a broad absorption peak at approximately 270 nm. This result highlights the importance of CNT purification in the study of optical properties of CNT.
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Cited by
Carbon nanotubes: properties, synthesis, purification, and medical applications
Eatemadi, A., Daraee, H., Karimkhanloo, H., Kouhi, M., Zarghami, N., Akbarzadeh, A., Abasi, M., Hanifehpour, Y., & Joo, S. W. (2014) · Nanoscale Research Letters · doi:10.1186/1556-276x-9-393 · Cited by 1,239
Recognition Ability of DNA for Carbon Nanotubes Correlates with Their Binding Affinity
Roxbury, D., Tu, X., Zheng, M., & Jagota, A. (2011) · Langmuir · doi:10.1021/la2007793 · Cited by 104
Self-citationStructural Characteristics of Oligomeric DNA Strands Adsorbed onto Single-Walled Carbon Nanotubes
Roxbury, D., Jagota, A., & Mittal, J. (2013) · The Journal of Physical Chemistry B · doi:10.1021/jp309523a · Cited by 59
Metadata
- Authors
- Huang, X., Mclean, R. S., & Zheng, M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-08-27
- Volume
- 77
- Issue
- 19
- Pages
- 6225-6228
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2030420118
- Cited by
- 246
- References
- 0
- FWCI
- 9.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huang, X., Mclean, R. S., & Zheng, M. (2005). High-Resolution Length Sorting and Purification of DNA-Wrapped Carbon Nanotubes by Size-Exclusion Chromatography. Analytical Chemistry. https://doi.org/10.1021/ac0508954
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