The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Detection of Alkaline Phosphatase Using Surface-Enhanced Raman Spectroscopy
Abstract
A new approach was developed to detect the activity of alkaline phosphatase (ALP) enzyme at ultralow concentrations using a surface-enhanced Raman scattering (SERS) technique. The approach is based on the use of gold nanoparticles as a SERS material whereas 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate of ALP. The enzymatic hydrolysis of BCIP led to the formation of indigo dye derivatives, which were found to be highly SERS active. For the first time, we were able to detect ALP at a concentration of approximately 4 x 10(-15) M or at single-molecule levels when ALP was incubated with BCIP for 1 h in the Tris-HCl buffer. The same technique also was successfully employed to detect surface-immobilized avidin, and a detection limit of 10 ng/mL was achieved. This new technique allows the detection of both free and labeled ALP as a Raman probe in enzyme immunoassays, immunoblotting, and DNA hybridization assays at ultralow concentrations.
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Citations
Cites
Surface-Enhanced Raman Spectroscopy
Haynes, C. L., McFarland, A. D., & Van Duyne, R. P. (2005) · Analytical Chemistry · doi:10.1021/ac053456d · Cited by 1,144
Cited by
Gold Nanoparticles in Chemical and Biological Sensing
Saha, K., Agasti, S. S., Kim, C., Li, X., & Rotello, V. M. (2012) · Chemical Reviews · doi:10.1021/cr2001178 · Cited by 4,620
Metadata
- Authors
- Ruan, C., Wang, W., & Gu, B.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-04-08
- Volume
- 78
- Issue
- 10
- Pages
- 3379-3384
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1972006179
- Cited by
- 254
- References
- 1
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- closed
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