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Cotton HILIC SPE Microtips for Microscale Purification and Enrichment of Glycans and Glycopeptides
Abstract
Solid-phase extraction microtips are important devices in modern bioanalytics, as they allow miniaturized sample preparation for mass spectrometric analysis. Here we introduce the use of cotton wool for the preparation of filter-free HILIC SPE microtips. To this end, pieces of cotton wool pads (approximately 500 μg) were packed into 10 μL pipet tips. The performance of the tips was evaluated for microscale purification of tryptic IgG Fc N-glycopeptides. Cotton wool HILIC SPE microtips allowed the removal of salts, most nonglycosylated peptides, and detergents such as SDS from glycoconjugate samples. MALDI-TOF-MS glycopeptide profiles were very repeatable with different tips as well as reused tips, and very similar profiles were obtained with different brands of cotton wool pads. In addition, we used cotton HILIC microtips to purify N-glycans after N-glycosidase F treatment of IgG and transferrin followed by MALDI-TOF-MS detection. In conclusion, we establish cotton wool microtips for glycan and glycopeptide purification with subsequent mass spectrometric detection.
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Cited by
Mass spectrometry approaches to glycomic and glycoproteomic analyses
Ruhaak, L. R., Xu, G., Li, Q., Goonatilleke, E., & Lebrilla, C. B. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00732 · Cited by 395
Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments
Kailemia, M. J., Ruhaak, L. R., Lebrilla, C. B., & Amster, I. J. (2014) · Analytical Chemistry · doi:10.1021/ac403969n · Cited by 377
Bondt, A., Rombouts, Y., Selman, M. H. J., Hensbergen, P. J., Reiding, K. R., Hazes, J. M. W., Dolhain, R. J. E. M., & Wuhrer, M. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m114.039537 · Cited by 264
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Metadata
- Authors
- Selman, M. H. J., Hemayatkar, M., Deelder, A. M., & Wuhrer, M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-04
- Volume
- 83
- Issue
- 7
- Pages
- 2492-2499
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2068980146
- Cited by
- 343
- References
- 0
- FWCI
- 5.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- Selman, M. H. J., Hemayatkar, M., Deelder, A. M., & Wuhrer, M. (2011). Cotton HILIC SPE Microtips for Microscale Purification and Enrichment of Glycans and Glycopeptides. Analytical Chemistry. https://doi.org/10.1021/ac1027116
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