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“Molecular Beacon”-Based Fluorescent Assay for Selective Detection of Glutathione and Cysteine
Abstract
We report on the development of a fluorescence turn-on "molecular beacon" probe for the detection of glutathione (GSH) and cysteine (Cys). The method is based on a competitive ligation of Hg(2+) ions by GSH/Cys and thymine-thymine (T-T) mismatches in a DNA strand of the self-hybridizing beacon strand. The assay relies on the distance-dependent optical properties of the fluorophore/quencher pair attached to the ends of the molecular beacon DNA strand. In a very selective coordination of Hg(2+) to GSH/Cys, the fluorophore/quencher distance increases concomitantly with the dehybridization and dissociation of the beacon stem T-Hg(2+)-T due to the extraction of Hg(2+) ions. This process results in switching the molecular beacon to the "on" state. The concentration range of the probe is 4-200 nM with the limit of detection (LOD) of 4.1 nM for GSH and 4.2 nM Cys. The probe tested satisfactorily against interference for a range of amino acids including sulfur-containing methionine.
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Drummond, T. G., Hill, M. G., & Barton, J. K. (2003) · Nature Biotechnology · doi:10.1038/nbt873 · Cited by 2,214
Chauhan, A., & Chauhan, V. (2006) · Pathophysiology · doi:10.1016/j.pathophys.2006.05.007 · Cited by 656
Cited by
Zhang, X.-L., Zheng, C., Guo, S.-S., Li, J., Yang, H.-H., & Chen, G. (2014) · Analytical Chemistry · doi:10.1021/ac500336f · Cited by 414
Glutathione: Antioxidant Properties Dedicated to Nanotechnologies
Gaucher, C., Boudier, A., Bonetti, J., Clarot, I., Leroy, P., & Parent, M. (2018) · Antioxidants · doi:10.3390/antiox7050062 · Cited by 332
Metadata
- Authors
- Xu, H., & Hepel, M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-01-06
- Volume
- 83
- Issue
- 3
- Pages
- 813-819
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2011034028
- Cited by
- 163
- References
- 2
- FWCI
- 8.93
- Type
- journal article
- Language
- en
- Open access
- closed
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