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Identification of Protein Remains in Archaeological Potsherds by Proteomics

Solazzo, Caroline, Fitzhugh, William W., Rolando, Christian, Tokarski, Caroline·2008·Analytical Chemistry
DOI 10.1021/ac800515v
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Abstract

We demonstrate here the possibility of identifying proteins trapped in few milligrams of the clay matrix of a 1200-1400 AD Iñupiat potsherd fragment from Point Barrow, Alaska, by a dedicated proteomics approach. The four main steps of a proteomics analysis, (i) protein extraction from biological samples, (ii) protein hydrolysis using a hydrolase enzyme, (iii) nanoLC, nanoESI MS, and MS/MS analysis of the generated peptides, and (iv) protein identification using protein databank proceeded from genomic data, have been optimized for archeological remains. Briefly our procedure starts by grinding the potsherds, extraction with 1% trifluoroacetic acid, digestion with excess of trypsin, nanoLC, nanoESI FT-ICR analysis, and data mining by homology search. The developed conditions were evaluated on protein extracts from remains obtained by heated muscle tissues and blubbers of different seal and whale species, these samples representing the main diet sources of the Eskimo population. Most of the proteins were identified by sequence homology to other species due to the lack of cetacean and pinniped proteins in the databanks. More interestingly, two proteins, myoglobin and hemoglobin, respectively, identified in muscle tissue samples and blubber samples highlight several specific peptides of cetacean and pinniped species; these peptides are significant to prove the presence of these marine species in the analyzed samples. Based on the developed methodology and on protein identification…

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  • While some success from extracting proteins from ceramics has been reported, challenges have arisen in removal of protein from the silica matrix and protein preservation in this context is not fully understood.

    “While some success from extracting proteins from ceramics has been reported (117–120), challenges have arisen in the removal of protein from the silica matrix and protein preservation in this context is not fully understood (7, 121, 122).”

    From Ancient protein analysis in archaeology (2021) · cites this paper as [119]

  • Mass spectrometry-based proteomics has already been used to analyze artifacts, archaeological clay matrices, and ancient food.

    “Indeed, mass spectrometry-based proteomics has already been used in a multi-omics approach to analyze artifacts [17], archaeological clay matrices [18], and ancient food [19-21].”

    From Metaproteomic analysis of King Ghezo tomb wall (Abomey, Benin) confirms 19th century voodoo sacrifices (2024) · cites this paper as [18]

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Research works printed but not linked: 29

  1. Garnier N., 2002, Anal. Chem., 74, 4868, 10.1021/ac025637a · DOI
  2. Regert M., 2002, Anal. Chem., 74, 965, 10.1021/ac0155862 · DOI
  3. Evershed R. P., 2002, Acc. Chem. Res., 35, 660, 10.1021/ar000200f · DOI
  4. Regert M., 2003, Meas. Sci. Technol., 14, 1620, 10.1088/0957-0233/14/9/313 · DOI
  5. Regert M., 2006, Anal. Chim. Acta, 577, 140, 10.1016/j.aca.2006.06.038 · DOI
  6. Martin E., 1977, Stud. Conserv., 22, 63, 10.1179/sic.1977.009 · DOI
  7. Jones P. L., 1962, Stud. Conserv., 7, 10, 10.1179/sic.1962.003 · DOI
  8. Johnson M., 1971, Stud. Conserv., 16, 145, 10.1179/sic.1971.017 · DOI
  9. Kockaert L., 1989, Stud. Conserv., 34, 183, 10.1179/sic.1989.34.4.183 · DOI
  10. Downs E. F., 1995, J. Archeol. Sci., 22, 11, 10.1016/S0305-4403(95)80160-X · DOI
  11. Craig O., 2000, Nature, 408, 312, 10.1038/35042684 · DOI · Paper page
  12. Casoli A., 1996, Chromatographia, 42, 421, 10.1007/BF02272134 · DOI
  13. Evershed R. P., 1996, J. Archeol. Sci., 23, 429, 10.1006/jasc.1996.0038 · DOI · Paper page
  14. Colombini M. P., 2004, J. Sep. Sci., 27, 147, 10.1002/jssc.200301625 · DOI
  15. Chen X., 2005, Can. J. Anal. Sci. Spectrosc., 50, 325
  16. Andreotti A., 2006, Anal. Chem., 78, 4490, 10.1021/ac0519615 · DOI
  17. Prikryl P., 2006, J. Sep. Sci., 29, 2653, 10.1002/jssc.200600171 · DOI
  18. Peris-Vicente J., 2006, Talanta, 68, 1648, 10.1016/j.talanta.2005.08.050 · DOI
  19. Schoeninger M. J., 1983, Science, 220, 1381, 10.1126/science.6344217 · DOI
  20. DeNiro M. J., 1985, Nature, 317, 806, 10.1038/317806a0 · DOI
  21. Scarabino C., 2006, Isotopes Environ. Health Stud., 42, 151, 10.1080/10256010600672926 · DOI
  22. Ostrom P. H., 2000, Geochim. Cosmochim. Acta, 64, 1043, 10.1016/S0016-7037(99)00381-6 · DOI
  23. Kuckova S., 2005, Anal. Bioanal. Chem., 382, 275, 10.1007/s00216-005-3108-5 · DOI
  24. Tokarski C., 2006, Anal. Chem., 78, 1494, 10.1021/ac051181w · DOI
  25. Ostrom P. H., 2006, Geochim. Cosmochim. Acta, 70, 2034, 10.1016/j.gca.2006.01.004 · DOI
  26. Asara J. M., 2007, Science, 316, 280, 10.1126/science.1137614 · DOI · Paper page
  27. Schweitzer M. H., 2007, Science, 316, 277, 10.1126/science.1138709 · DOI
  28. Barnard H., 2007, J. Archaeol. Sci., 34, 28, 10.1016/j.jas.2006.03.010 · DOI
  29. Solazzo C., 2007, Br. Archaeol. Rep., 13, 161

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Metadata

Authors
Solazzo, C., Fitzhugh, W. W., Rolando, C., & Tokarski, C.
Affiliations
Chimie Organique et Macromoléculaire, UMR CNRS 8009, and Protéomique, Modifications Post-traductionnelles et Glycobiologie, IFR 147, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq Cedex, France, and Department of Anthropology, Smithsonian Institution, MRC 112, Washington, D.C. 20013-7012
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2008-05-22
Volume
80
Issue
12
Pages
4590-4597
ISSN
  • 0003-2700
  • 1520-6882
DOI
10.1021/ac800515v
Cited by
130
References
0
Language
en

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