The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Development of a Robust and Repeatable UPLC−MS Method for the Long-Term Metabolomic Study of Human Serum
Abstract
A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance Liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS). This method was specifically designed to fulfill the requirements of a long-term metabolomic study, spanning more than 3 years, and it was subsequently thoroughly evaluated for robustness and repeatability. We describe here the observed drift in instrumental performance over time and its improvement with adjustment of the length of analytical block. The optimal setup for our purpose was further validated against a set of serum samples from 30 healthy individuals. We also assessed the reproducibility of chromatographic columns with the same chemistry of stationary phase from the same manufacturer but from different production batches. The results have allowed the authors to prepare SOPs for "fit for purpose" long-term UPLC-MS metabolomic studies, such as are being employed in the HUSERMET project. This method allows the acquisition of data and subsequent comparison of data collected across many months or years.
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Cites
Kell, D. B., & Oliver, S. G. (2004) · BioEssays · doi:10.1002/bies.10385 · Cited by 535
Self-citationGrowth control of the eukaryote cell: a systems biology study in yeast
Castrillo, J. I., Zeef, L. A., Hoyle, D. C., Zhang, N., Hayes, A., Gardner, D. C., Cornell, M. J., Petty, J., Hakes, L., Wardleworth, L., et al. (2007) · Journal of Biology · doi:10.1186/jbiol54 · Cited by 263
Self-citationAtherton, H. J., Bailey, N. J., Zhang, W., Taylor, J., Major, H., Shockcor, J., Clarke, K., & Griffin, J. L. (2006) · Physiological Genomics · doi:10.1152/physiolgenomics.00060.2006 · Cited by 159
Cited by
The Human Serum Metabolome (HUSERMET) Consortium, Dunn, W. B., Broadhurst, D., Begley, P., Zelena, E., Francis-McIntyre, S., Anderson, N., Brown, M., Knowles, J. D., Halsall, A., et al. (2011) · Nature Protocols · doi:10.1038/nprot.2011.335 · Cited by 3,294
Self-citationA community-driven global reconstruction of human metabolism
Thiele, I., Swainston, N., Fleming, R. M. T., Hoppe, A., Sahoo, S., Aurich, M. K., Haraldsdottir, H., Mo, M. L., Rolfsson, O., Stobbe, M. D., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2488 · Cited by 1,019
Self-citationSelf-Driving Laboratories for Chemistry and Materials Science
Tom, G., Schmid, S. P., Baird, S. G., Cao, Y., Darvish, K., Hao, H., Lo, S., Pablo-García, S., Rajaonson, E. M., Skreta, M., et al. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00055 · Cited by 707
Determinants of accelerated metabolomic and epigenetic aging in a UK cohort
Robinson, O., Chadeau Hyam, M., Karaman, I., Climaco Pinto, R., Ala-Korpela, M., Handakas, E., Fiorito, G., Gao, H., Heard, A., Jarvelin, M., et al. (2020) · Aging Cell · doi:10.1111/acel.13149 · Cited by 205
Masson, P., Alves, A. C., Ebbels, T. M. D., Nicholson, J. K., & Want, E. J. (2010) · Analytical Chemistry · doi:10.1021/ac101722e · Cited by 173
Metadata
- Authors
- Zelena, E., Dunn, W. B., Broadhurst, D., Francis-McIntyre, S., Carroll, K. M., Begley, P., O’Hagan, S., Knowles, J. D., Halsall, A., HUSERMET Consortium, Wilson, I. D., & Kell, D. B.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-01-26
- Volume
- 81
- Issue
- 4
- Pages
- 1357-1364
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2088716662
- Cited by
- 811
- References
- 3
- FWCI
- 12.48
- Type
- journal article
- Language
- en
- Open access
- closed
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