The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Convenient and Continuous Fluorometric Assay Method for Acetylcholinesterase and Inhibitor Screening Based on the Aggregation-Induced Emission
Abstract
A new convenient and continuous fluorometric assay method for acetylcholinesterase (AChE) and its inhibitor screening is successfully established with the ensemble of 1 [a TPE (tetraphenylethylene) compound with two sulfonate (-SO(3)(-)) units] and myristoylcholine (an amphiphilic compound as a good substrate of AChE). This new assay method is designed by making use of the aggregation-induced emission (AIE) feature of TPE compounds. Both dynamic light scattering (DLS) and fluorescence confocal microscopic measurements indicated the formation of aggregation complex for the ensemble of 1 and myristoylcholine and further disassembly of the aggregation complex after introducing AChE. The analysis for AChE can be carried out continuously, and AChE with concentration as low as 0.5 U/mL can be assayed. The results also clearly demonstrate the usefulness of this convenient assay method for kinetic study of AChE-catalyzed myristoylcholine hydrolysis and screening inhibitors of AChE. Given its simplicity and easy operation, this method may extend to high-throughput screening of AChE inhibitors and relevant Alzheimer's disease (AD) drug discovery.
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Cites
Alzheimer's Disease and Senile Dementia: Loss of Neurons in the Basal Forebrain
Whitehouse, P., Price, D., Struble, R., Clark, A., Coyle, J., & DeLong, M. R. (1982) · Science · doi:10.1126/science.7058341 · Cited by 3,627
Cited by
Aggregation-Induced Emission: Together We Shine, United We Soar!
Mei, J., Leung, N. L. C., Kwok, R. T. K., Lam, J. W. Y., & Tang, B. Z. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00263 · Cited by 8,270
Wang, M., Zhang, G., Zhang, D., Zhu, D., & Tang, B. Z. (2010) · Journal of Materials Chemistry · doi:10.1039/b921610c · Cited by 852
Self-citationAcetylcholinesterase inhibition-based biosensors for pesticide determination: A review
Pundir, C. S., & Chauhan, N. (2012) · Analytical Biochemistry · doi:10.1016/j.ab.2012.06.025 · Cited by 349
Hu, F., Huang, Y., Zhang, G., Zhao, R., Yang, H., & Zhang, D. (2014) · Analytical Chemistry · doi:10.1021/ac502103t · Cited by 285
Self-citationDong, X., Zhang, G., Shi, J., Wang, Y., Wang, M., Peng, Q., & Zhang, D. (2017) · Chem. Commun. · doi:10.1039/c7cc07092f · Cited by 54
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Metadata
- Authors
- Wang, M., Gu, X., Zhang, G., Zhang, D., & Zhu, D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-04-17
- Volume
- 81
- Issue
- 11
- Pages
- 4444-4449
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2026058961
- Cited by
- 251
- References
- 1
- FWCI
- 14.94
- Type
- journal article
- Language
- en
- Open access
- closed
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