The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Label-Free Highly Sensitive Detection of Proteins in Aqueous Solutions Using Surface-Enhanced Raman Scattering
Abstract
We detected concentration-dependent surface-enhanced Raman scattering (SERS) spectra of several label-free proteins (lysozyme, ribonuclease B, avidin, catalase, and hemoglobin) for the first time in aqueous solutions. Acidified sulfate was used as an aggregation agent to induce high electromagnetic enhancement in SERS. Strong SERS spectra of simple and conjugated protein samples could easily be accessed after the pretreatment with the aggregation agent. The detection limits of the proposed method for lysozyme and catalase were as low as 5 microg/mL and 50 ng/mL, respectively. This detection protocol for label-free proteins has combined simplicity, sensitivity, and reproducibility and allows routine qualitative and relatively quantitative detections. Thus, it has great potential in practical high-throughput protein detections.
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Cites
Laser-excited Raman spectroscopy of biomolecules
Lord, R. C., & Yu, N.-T. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90208-1 · Cited by 366
Cited by
SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging
Lane, L. A., Qian, X., & Nie, S. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00265 · Cited by 873
Surface-enhanced Raman spectroscopy: a half-century historical perspective
Yi, J., You, E.-M., Hu, R., Wu, D.-Y., Liu, G.-K., Yang, Z.-L., Zhang, H., Gu, Y., Wang, Y.-H., Wang, X., et al. (2025) · Chemical Society Reviews · doi:10.1039/d4cs00883a · Cited by 382
Self-citationQuantitative volumetric Raman imaging of three dimensional cell cultures
Kallepitis, C., Bergholt, M. S., Mazo, M. M., Leonardo, V., Skaalure, S. C., Maynard, S. A., & Stevens, M. M. (2017) · Nature Communications · doi:10.1038/ncomms14843 · Cited by 164
Metadata
- Authors
- Han, X. X., Huang, G. G., Zhao, B., & Ozaki, Y.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-03-27
- Volume
- 81
- Issue
- 9
- Pages
- 3329-3333
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2090239693
- Cited by
- 221
- References
- 1
- FWCI
- 7.40
- Type
- journal article
- Language
- en
- Open access
- closed
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