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Aptamer−DNAzyme Hairpins for Amplified Biosensing
Abstract
Engineered nucleic acid hairpin structures are used for the amplified analysis of low-molecular-weight substrates (adenosine monophosphate, AMP) or proteins (lysozyme). The hairpin structures consist of the anti-AMP or antilysozyme aptamer units linked to the horseradish peroxidase (HRP)-mimicking DNAzyme sequence. The HRP-mimicking DNAzyme sequence is protected in a "caged", inactive structure in the stem regions of the respective hairpins, whereas the loop regions include a part of the respective aptamer sequence. The opening of the hairpins by the analytes, AMP or lysozyme, through the formation of the respective analyte-aptamer complexes, results in the self-assembly of the active HRP-mimicking DNAzyme. The DNAzyme catalyzes the H(2)O(2)-mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(2-)) to the colored ABTS(*-), thus providing the amplified optical detection of the respective analytes. The engineered aptamer-DNAzyme hairpin structures reveal significantly improved analytical performance, as compared to analogous fluorophore-quencher-labeled hairpins.
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Citations
Cited by
Wang, F., Lu, C.-H., & Willner, I. (2014) · Chemical Reviews · doi:10.1021/cr400354z · Cited by 649
Self-citationAmplified Analysis of DNA by the Autonomous Assembly of Polymers Consisting of DNAzyme Wires
Wang, F., Elbaz, J., Orbach, R., Magen, N., & Willner, I. (2011) · Journal of the American Chemical Society · doi:10.1021/ja2076789 · Cited by 339
Self-citationOptical aptasensors for quantitative detection of small biomolecules: A review
Feng, C., Dai, S., & Wang, L. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.03.014 · Cited by 300
A general approach to the design of allosteric, transcription factor-regulated DNAzymes
Adornetto, G., Porchetta, A., Palleschi, G., Plaxco, K. W., & Ricci, F. (2015) · Chemical Science · doi:10.1039/c5sc00228a · Cited by 31
Metadata
- Authors
- Teller, C., Shimron, S., & Willner, I.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-09-25
- Volume
- 81
- Issue
- 21
- Pages
- 9114-9119
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2017815617
- Cited by
- 199
- References
- 0
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- Teller, C., Shimron, S., & Willner, I. (2009). Aptamer−DNAzyme Hairpins for Amplified Biosensing. Analytical Chemistry. https://doi.org/10.1021/ac901773b
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