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Comprehensive On-Line LC/LC/MS of Proteins
Abstract
This is a description of a comprehensive two-dimensional liquid chromatography (LC) system for the separation of protein mixtures. This system uses cation-exchange chromatography followed by reversed-phase chromatography (RPLC). The two LC systems are coupled by an eight-port valve equipped with two storage loops and under computer control. The RPLC effluent is sampled by both a UV detector and an electrospray mass spectrometer. In this way, complex mixtures of large biomolecules can be rapidly separated, desalted, and analyzed for molecular weight in less than 2 h. The system's utility is demonstrated with a mixture of standards and an Escherichia coli cell lysate.
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Cited by
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., & Aebersold, R. (1999) · Nature Biotechnology · doi:10.1038/13690 · Cited by 4,683
Direct analysis of protein complexes using mass spectrometry
Link, A. J., Eng, J., Schieltz, D. M., Carmack, E., Mize, G. J., Morris, D. R., Garvik, B. M., & Yates, J. R. (1999) · Nature Biotechnology · doi:10.1038/10890 · Cited by 2,237
Mass Spectrometry in Proteomics
Aebersold, R., & Goodlett, D. R. (2001) · Chemical Reviews · doi:10.1021/cr990076h · Cited by 1,238
Proteomics by Mass Spectrometry: Approaches, Advances, and Applications
Yates, J. R., Ruse, C. I., & Nakorchevsky, A. (2009) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-061008-124934 · Cited by 1,078
Proteomics: the move to mixtures
Peng, J., & Gygi, S. P. (2001) · Journal of Mass Spectrometry · doi:10.1002/jms.229 · Cited by 648
Metadata
- Authors
- Opiteck, G. J., Lewis, K. C., Jorgenson, J. W., & Anderegg, R. J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-04-01
- Volume
- 69
- Issue
- 8
- Pages
- 1518-1524
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2052920249
- Cited by
- 286
- References
- 0
- FWCI
- 5.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Comprehensive On-Line LC/LC/MS of Proteins. https://app.cochat.ai/papers/10.1021/ac961155l
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- Opiteck, G. J., Lewis, K. C., Jorgenson, J. W., & Anderegg, R. J. (1997). Comprehensive On-Line LC/LC/MS of Proteins. Analytical Chemistry. https://doi.org/10.1021/ac961155l
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
