The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Minisonicator To Rapidly Disrupt Bacterial Spores for DNA Analysis
Abstract
Concerns about the use of anthrax spores as a weapon of mass destruction have motivated the development of portable instruments capable of detecting and monitoring a suspected release of the agent. Optimal detection of bacterial spores by PCR requires that the spores be disrupted to make the endogenous DNA available for amplification. The entire process of spore lysis, PCR, and detection can take several hours using conventional methods and instruments. In this report, a minisonicator and prototype spore lysis cartridge were built to disrupt Bacillus spores in 30 s for rapid, real-time PCR analysis. Utilization of the minisonicator improved PCR analysis by decreasing the limit of detection, reducing the time of detection, and increasing the signal amplitude. Total time of spore disruption and detection using the minisonicator and a microchip PCR instrument was less than 15 min.
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Micro Total Analysis Systems. 2. Analytical Standard Operations and Applications
Auroux, P.-A., Iossifidis, D., Reyes, D. R., & Manz, A. (2002) · Analytical Chemistry · doi:10.1021/ac020239t · Cited by 1,532
Microfluidic chips for clinical and forensic analysis
Verpoorte, E. (2002) · ELECTROPHORESIS · doi:10.1002/1522-2683(200203)23:5<677::aid-elps677>3.0.co;2-8 · Cited by 526
Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
Lim, D. V., Simpson, J. M., Kearns, E. A., & Kramer, M. F. (2005) · Clinical Microbiology Reviews · doi:10.1128/cmr.18.4.583-607.2005 · Cited by 417
Metadata
- Authors
- Belgrader, P., Hansford, D., Kovacs, G. T. A., Venkateswaran, K., Mariella, Milanovich, F., Nasarabadi, S., Okuzumi, M., Pourahmadi, F., & Northrup, M. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-08-31
- Volume
- 71
- Issue
- 19
- Pages
- 4232-4236
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1998095407
- Cited by
- 170
- References
- 1
- FWCI
- 2.07
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
