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ProFound: An Expert System for Protein Identification Using Mass Spectrometric Peptide Mapping Information
Abstract
We describe the protein search engine "ProFound", which employs a Bayesian algorithm to identify proteins from protein databases using mass spectrometric peptide mapping data. The algorithm ranks protein candidates by taking into account individual properties of each protein in the database as well as other information relevant to the peptide mapping experiment. The program consistently identifies the correct protein(s) even when the data quality is relatively low or when the sample consists of a simple mixture of proteins. Illustrative examples of protein identifications are provided.
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Gelman, A., Carlin, J. B., Stern, H. S., & Rubin, D. B. (2003) · doi:10.1201/9780429258480 · Cited by 27,276
Cited by
A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry
Nesvizhskii, A. I., Keller, A., Kolker, E., & Aebersold, R. (2003) · Analytical Chemistry · doi:10.1021/ac0341261 · Cited by 4,809
Proteomics: the move to mixtures
Peng, J., & Gygi, S. P. (2001) · Journal of Mass Spectrometry · doi:10.1002/jms.229 · Cited by 648
Friso, G., Giacomelli, L., Ytterberg, A. J., Peltier, J.-B., Rudella, A., Sun, Q., & Wijk, K. J. V. (2004) · The Plant Cell · doi:10.1105/tpc.017814 · Cited by 482
The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes
Berwick, D. C., Hers, I., Heesom, K. J., Moule, S. K., & Tavareá, J. M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204681200 · Cited by 383
Thiede, B., Höhenwarter, W., Krah, A., Mattow, J., Schmid, M., Schmidt, F., & Jungblut, P. R. (2005) · Methods · doi:10.1016/j.ymeth.2004.08.015 · Cited by 278
Metadata
- Authors
- Zhang, W., & Chait, B. T.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-05-04
- Volume
- 72
- Issue
- 11
- Pages
- 2482-2489
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1985560593
- Cited by
- 595
- References
- 1
- FWCI
- 22.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhang, W., & Chait, B. T. (2000). ProFound: An Expert System for Protein Identification Using Mass Spectrometric Peptide Mapping Information. Analytical Chemistry. https://doi.org/10.1021/ac991363o
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.