Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques

Fornelli, Luca, Srzentić, Kristina, Huguet, Romain, Mullen, Christopher, Sharma, Seema, Zabrouskov, Vlad, Fellers, Ryan T., Durbin, Kenneth R. +2 more·2018·Analytical Chemistry

Cited by (150)

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  5. Intact Mass Profiling Reveals Phospho‐Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility
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  10. Development of Top-Down Mass Spectrometry Strategies in the Chromatographic Time Scale (LC-TD-MS) for the Extended Characterization of an Anti-EGFR Single-Domain Antibody-Drug Conjugate in Both Reduced and Nonreduced Forms
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  11. Comparative Analysis of Proteoform Clean‐Up Methods for In‐Depth Top‐Down Proteomics
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  12. Characterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry
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  13. TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics
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  14. Sequencing a Bispecific Antibody by Controlling Chain Concentration Effects When Using an Immobilized Nonspecific Protease
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  15. Ion Activation Methods for Top‐Down Proteomics
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  16. A Prototype timsOmni Platform Enables Confident Annotation of the Key Hypervariable CDR3 Regions of IgG Immunoglobulins Using Low- and High-Energy Electron-Based Fragmentation
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  17. Automating Middle-Down Mass Spectrometry Analysis for Extensive Antibody Characterization
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  18. From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization
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  19. Therapeutic monoclonal antibodies in the central nervous system: Pharmacokinetics, bioanalysis and clinical implications
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  20. Diagnostic utility of N-terminal TMPP labels for unambiguous identification of clipped sites in therapeutic proteins
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  21. Revealing Unpredicted Aspartic Acid Isomerization Hotspots by Probing Diagnostic Fragmentation Propensities in Top-Down and Middle-Down Mass Spectrometry
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  23. Effective Amino Acid Sequencing of Intact Filgrastim by Multimodal Mass Spectrometry with Topdownr.
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  24. Gábor Transform-Based Antibody Quantitation in Serum: An Interlaboratory Liquid Chromatography/High-Resolution Mass Spectrometry Investigation
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  28. Parallel Sequencing of the Two Arms on a Bispecific Antibody by Electron Capture Dissociation on a timsOmni Platform Reveals Several By-products in the Controlled Fab-arm Exchange Process
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  29. Native Top-Down Mass Spectrometry Combined with High-Resolution Charge Variant Analysis of Trastuzumab Originator and Biosimilars
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  30. Middle-Down Mass Spectrometry Characterization of Monoclonal Antibody Enabled by Electron-Activated Dissociation on a Time-of-Flight Platform: A Case Study of Methionine Oxidation PTM Identification and Quantification
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  31. Antibody proteomics
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  32. Internal Fragment Ions from Higher Energy Collision Dissociation Enable the Glycoform-Resolved Asn325 Deamidation Assessment of Antibodies by Middle-Down Mass Spectrometry
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  33. Proteo-SAFARI: An R Application for Identification of Fragment Ions in Top–Down MS/MS Spectra of Proteins
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  34. Combination of hydrophilic interaction liquid chromatography and top-down mass spectrometry for characterisation of adeno-associated virus capsid proteins
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  35. Integration of liquid chromatography mass spectrometry with a heavy peptide response curve accurately measures unprocessed C-terminal lysine during peptide mapping analysis of therapeutic antibodies in a single run
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  36. Using TDFragMapper: a high-performance tool to assess experimental parameters in top-down proteomics
    Diogo Borges Lima, Jonathan Dhenin, Mathieu Dupré, Julia Chamot‐Rooke · 2021 · Research Square
  37. Development of fine-tuned top-down mass spectrometry strategies in the chromatographic time scale (LC-TD-MS) for the complete characterization of an anti EGFR single domain antibody-drug conjugate (sdADC)
    Rania Benazza, Greg Papadakos, Jen Thom, Hélène Diemer, Graham Cotton, Sarah Cianférani, Oscar Hernandez‐Alba · 2024 · ChemRxiv
  38. A prototype timsOmni platform enables confident annotation of key hypervariable regions of IgG immunoglobulins using low- and high-energy electron-based fragmentation
    Simon Ollivier, Dina Schuster, Danique M.H. van Rijswijck, Iuliia Stroganova, Athanasios Smyrnakis, Jan Fiala, Mariangela Kosmopoulou, Detlev Suckau, Stuart Pengelley, Oliver Raether et al. · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  39. High‐Performance Liquid Chromatography–Mass Spectrometry in Peptide and Protein Analysis
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  41. Bioanalytical methodology to study the in vivo biotransformation of therapeutic proteins
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  45. Intact Mass Profiling Reveals Phospho‐Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility
    Che‐Fan Huang, Taojunfeng Su, Annette S. Flozak, Cara J. Gottardi, Neil L. Kelleher · 2025 · Angewandte Chemie
  46. Middle‐Down Nuclear Magnetic Resonance for Therapeutic Monoclonal Antibody Glycan Profiling
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  48. Establishment of a Top‐Down Liquid Chromatography‐High‐Resolution‐Mass Spectrometry Method With Multiple Fragmentation Modes for Clinical Diagnosis of Hemoglobin Variants
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  49. Development of new methodologies for native mass spectrometry and ion mobility approaches to characterize multiprotein complexes and therapeutic proteins
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  50. Electron activated dissociation technique for middle-down mass spectrometry analysis of disulphide bonds and free thiols: Application in characterising monoclonal antibody biotherapeutic fragments
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