Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
Fornelli, Luca, Srzentić, Kristina, Huguet, Romain, Mullen, Christopher, Sharma, Seema, Zabrouskov, Vlad, Fellers, Ryan T., Durbin, Kenneth R. +2 more·2018·Analytical Chemistry
Cited by (150)
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- A Prototype timsOmni Platform Enables Confident Annotation of the Key Hypervariable CDR3 Regions of IgG Immunoglobulins Using Low- and High-Energy Electron-Based FragmentationSimon Ollivier, Dina Schuster, Danique M.H. van Rijswijck, Iuliia Stroganova, Athanasios Smyrnakis, Jan Fiala, Mariangela Kosmopoulou, Detlev Suckau, Stuart Pengelley, Oliver Raether et al. · 2026 · Analytical Chemistry
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- Revealing Unpredicted Aspartic Acid Isomerization Hotspots by Probing Diagnostic Fragmentation Propensities in Top-Down and Middle-Down Mass SpectrometryBlandine Denefeld, Joanna Hajduk, Jure Cerar, Jean‐Michel Rondeau, Jérôme Dayer, Manuel Lang, Wolfram Kern, François Griaud · 2025 · Journal of the American Society for Mass Spectrometry
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- Sequential Ion-Ion Reactions for Enhanced Gas-Phase Sequencing of Large Intact Proteins in a Tribrid Orbitrap Mass SpectrometerJake T. Kline, Christopher Mullen, Kenneth R. Durbin, Ryan N. Oates, Romain Huguet, John E. P. Syka, Luca Fornelli · 2021 · ChemRxiv
- Enhancing the sequence coverage of nanodiamond-extracted early secretory proteins from the Mycobacterium tuberculosis complexPo‐Chi Soo, Ching-Chieh Lee, Meng-Fu Shie, Avinash A. Patil, Mhikee Janella N. Descanzo, Ya-Ching Chin, Hsi-An Chen, Yu‐Tze Horng, Chih‐Bin Lin, Jen‐Jyh Lee et al. · 2024 · Analytical Methods
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- Native Top-Down Mass Spectrometry Combined with High-Resolution Charge Variant Analysis of Trastuzumab Originator and BiosimilarsCorentin Beaumal, Kristina Srzentić, Sara Carillo, Jonathan Bones · 2026 · Journal of the American Society for Mass Spectrometry
- Middle-Down Mass Spectrometry Characterization of Monoclonal Antibody Enabled by Electron-Activated Dissociation on a Time-of-Flight Platform: A Case Study of Methionine Oxidation PTM Identification and QuantificationAbdulafeez Akinloye, Leigh Donnellan, Parul Mittal, Clifford Young, Mariam Nassiri, Mark R. Condina, Nathan Edwards, Alok Kishorkumar Shah, Peter Hoffmann · 2026 · Analytical Chemistry
- Antibody proteomicsL.Sh. Kazieva, Tatiana E. Farafonova, Victor Gavrilovich Zgoda · 2023 · Biomeditsinskaya Khimiya
- Internal Fragment Ions from Higher Energy Collision Dissociation Enable the Glycoform-Resolved Asn325 Deamidation Assessment of Antibodies by Middle-Down Mass SpectrometrySteffen Lippold, Lance Cadang, Tilman Schlothauer, Feng Yang · 2023 · Analytical Chemistry
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- Using TDFragMapper: a high-performance tool to assess experimental parameters in top-down proteomicsDiogo Borges Lima, Jonathan Dhenin, Mathieu Dupré, Julia Chamot‐Rooke · 2021 · Research Square
- Development of fine-tuned top-down mass spectrometry strategies in the chromatographic time scale (LC-TD-MS) for the complete characterization of an anti EGFR single domain antibody-drug conjugate (sdADC)Rania Benazza, Greg Papadakos, Jen Thom, Hélène Diemer, Graham Cotton, Sarah Cianférani, Oscar Hernandez‐Alba · 2024 · ChemRxiv
- A prototype timsOmni platform enables confident annotation of key hypervariable regions of IgG immunoglobulins using low- and high-energy electron-based fragmentationSimon Ollivier, Dina Schuster, Danique M.H. van Rijswijck, Iuliia Stroganova, Athanasios Smyrnakis, Jan Fiala, Mariangela Kosmopoulou, Detlev Suckau, Stuart Pengelley, Oliver Raether et al. · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- High‐Performance Liquid Chromatography–Mass Spectrometry in Peptide and Protein AnalysisTun‐Li Shen · 2021 · Wiley eBooks
- Probing highly polymorphic proteins by mass spectrometryMax Hoek · 2022
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- All-ions laser induced dissociation (AI-LID) in the C-trap of a Q Exactive: Data independent identification and quantification directly from ultra-high resolution differential mass spectraLuke MacAleese, Xavier Dagany, Lény Garcia, Jérôme Lemoine, Philippe Dugourd, Marion Girod · 2025 · International Journal of Mass Spectrometry
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- Intact Mass Profiling Reveals Phospho‐Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin ContractilityChe‐Fan Huang, Taojunfeng Su, Annette S. Flozak, Cara J. Gottardi, Neil L. Kelleher · 2025 · Angewandte Chemie
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- Electron activated dissociation technique for middle-down mass spectrometry analysis of disulphide bonds and free thiols: Application in characterising monoclonal antibody biotherapeutic fragmentsAbdulafeez Akinloye, Leigh Donnellan, Clifford Young, Parul Mittal, Mark R. Condina, Peter Hoffmann · 2026 · Journal of Pharmaceutical and Biomedical Analysis