TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set

Thompson, Andrew; Wölmer, Nikolai; Koncarevic, Sasa; Selzer, Stefan; Böhm, Gitte; Legner, Harald; Schmid, Peter; Kienle, Stefan; Penning, Petra; Höhle, Claudia; Berfelde, Antje; Martinez-Pinna, Roxana; Farztdinov, Vadim; Jung, Stephan; Kuhn, Karsten; Pike, Ian

doi:10.1021/acs.analchem.9b04474

TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set

The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. The new reporter …