Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokine and gut microbiota modulation
Open accessAbstract
Dietary supplementation with conjugated linoleic acid (CLA) has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates dextran sulfate sodium (DSS)-induced colitis in mice. CLA was administered in five different doses: 40, 20, 10, 5, and 2.5 mg/day. Doses of CLA at 10 mg/day and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40, 20, and 10 mg/day CLA significantly increased the concentration of mucin2 and goblet cells, but neither 5 mg/day CLA nor 2.5 mg/day CLA had any effects. Meanwhile, 40 and 20 mg/day CLA treatments significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative-stress-related enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and inflammatory cytokines [tumor necrosis factor-α, interleukin (IL)-10, and IL-6] were modulated by 40 and 20 mg/day CLA. Furthermore, 40 mg/day CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter . In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier, and reverting microbiota changes.
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CLA treatment significantly increased the expression of the TJ proteins ZO-1, OCLN, and CLDN-3 in the DSS-induced colitis mouse model.
“Additionally, treatment with CLA significantly increased the expression of the TJ proteins ZO-1, OCLN, and CLDN-3 in the DSS-induced colitis mouse model.120”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [120]
Dietary supplementation of CLA ameliorated DSS-induced colitis in mice.
“Dietary supplementation of CLA ameliorated DSS-induced colitis in mice.120”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [120]
CLA treatment reduced the prevalence of Bacteroides and increased Bifidobacterium and Odoribacter in the DSS-induced colitis mouse model.
“Importantly, CLA treatment reduced the prevalence of Bacteroides and increased Bifidobacterium and Odoribacter in this model.”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [120]
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References 72 · Cited by 185
Research works printed but not linked: 3
- Pearson, C. Inflammatory bowel disease. Clin. Adv. Nutr. 2004, 100 (9), 86-90.
- Biondo-Simões, M. D. L. P.; Mandelli, K. K.; Pereira, M. A. C.; Faturi, J. L. Opções terapêuticas para as doenças inflamatórias intestinais: revisão. Rev. Bras. Coloproct. 2003, 23 (3), 172-182.
- Bassaganya-Riera, J.; Reynolds, K.; Martino-Catt, S.; Cui, Y.; Hennighausen, L.; Gonzalez, F.; Hontecillas, R. Activation of PPAR γ and δ by conjugated linoleic acid mediates protection from experimental inflammatory bowel disease. Gastroenterology 2004, 127 (3), 777-791.
- Authors
- Chen, Y., Yang, B., Ross, R. P., Jin, Y., Stanton, C., Zhao, J., Zhang, H., & Chen, W.
- Affiliations
- Jiangnan University (CN)
- Wuxi No.2 People's Hospital (CN)
- Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China
- Beijing Technology and Business University (CN)
- Corresponding author
- Yang, Bo
- Stanton, Catherine
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-11-06
- Volume
- 67
- Issue
- 48
- Pages
- 13282-13298
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2985976802
- Cited by
- 185
- References
- 72
- Citations by year
- 2026: 23 · 2025: 20 · 2024: 34 · 2023: 24 · 2022: 30 · 2021: 42 · 2020: 12
- FWCI
- 12.66
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Cork Open Research Archive (University College Cork, Ireland) (acceptedVersion)
- Arrow@dit (Dublin Institute of Technology) (submittedVersion)
- Funders
- National Natural Science Foundation of China (Nos. 31722041, Nos. 31801521)
- Fundamental Research Funds for the Central Universities (No. JUSRP51702A)
- Wuxi Young Talent Foundation (QNRC075)
- Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX19_1829)
- National First-Class Discipline Program of Food Science and Technology (JUFSTR20180102)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Keywords
- conjugated linoleic acid, DSS-induced colitis, inflammatory bowel disease, intestinal barrier, tight junction proteins, oxidative stress, inflammatory cytokines, gut microbiota, MUC2, goblet cells, epithelial apoptosis, dose-dependent effects, dietary supplementation
- MeSH terms
- Gastrointestinal Microbiome, Animals, Bacteria, Colitis, Humans, Intestinal Mucosa, Male, Mice, Inbred C57BL, Cytokines, Dextran Sulfate, Oxidative Stress, Linoleic Acids, Conjugated, Mice
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