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Responses of Pseudomonas putida to Zinc Excess Determined at the Proteome Level: Pathways Dependent and Independent of ColRS

Mumm, Karl, Ainsaar, Kadi, Kasvandik, Sergo, Tenson, Tanel, Hõrak, Rita·2016·Journal of Proteome Research
DOI 10.1021/acs.jproteome.6b00420
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Abstract

Zinc is an important micronutrient for bacteria, but its excess is toxic. Recently, the ColRS two-component system was shown to detect and respond to zinc excess and to contribute to zinc tolerance of Pseudomonas putida. Here, we applied a label-free whole-cell proteome analysis to compare the zinc-induced responses of P. putida and colR knockout. We identified dozens of proteins that responded to zinc in a ColR-independent manner, among others, known metal efflux systems CzcCBA1, CzcCBA2, CadA2 and CzcD. Nine proteins were affected in a ColR-dependent manner, and besides known ColR targets, four new candidates for ColR regulon were identified. Despite the relatively modest ColR-dependent changes of wild-type, colR deficiency resulted in drastic proteome alterations, with 122 proteins up- and 62 down-regulated by zinc. This zinc-promoted response had remarkable overlap with the alternative sigma factor AlgU-controlled regulon in P. aeruginosa. The most prominent hallmark was a high induction of alginate biosynthesis proteins and regulators. This response likely alleviates the zinc stress, as the AlgU-regulated alginate regulator AmrZ was shown to contribute to zinc tolerance of colR knockout. Thus, the ColRS system is important for zinc homeostasis, and in its absence, alternative stress response pathways are activated to support the zinc tolerance.

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Metadata

Authors
Mumm, K., Ainsaar, K., Kasvandik, S., Tenson, T., & Hõrak, R.
Affiliations
University of Tartu , , ,
Venue
Journal of Proteome Research
Publisher
American Chemical Society (ACS)
Published
2016-10-31
Volume
15
Issue
12
Pages
4349-4368
ISSN
  • 1535-3893
  • 1535-3907
DOI
10.1021/acs.jproteome.6b00420
Cited by
19
References
5
Language
en
Licence
Funders
Haridus- ja Teadusministeerium (IUT20-19, IUT2-22)

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