The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Antifouling and Antibacterial Multifunctional Polyzwitterion/Enzyme Coating on Silicone Catheter Material Prepared by Electrostatic Layer-by-Layer Assembly
Abstract
The formation of bacterial biofilms on indwelling medical devices generally causes high risks for adverse complications such as catheter-associated urinary tract infections. In this work, a strategy for synthesizing innovative coatings of poly(dimethylsiloxane) (PDMS) catheter material, using layer-by-layer assembly with three novel functional polymeric building blocks, is reported, i.e., an antifouling copolymer with zwitterionic and quaternary ammonium side groups, a contact biocidal derivative of that polymer with octyl groups, and the antibacterial hydrogen peroxide (H2O2) producing enzyme cellobiose dehydrogenase (CDH). CDH oxidizes oligosaccharides by transferring electrons to oxygen, resulting in the production of H2O2. The design and synthesis of random copolymers which combine segments that have antifouling properties by zwitterionic groups and can be used for electrostatically driven layer-by-layer (LbL) assembly at the same time were based on the atom-transfer radical polymerization of dimethylaminoethyl methacrylate and subsequent partial sulfobetainization with 1,3-propane sultone followed by quaternization with methyl iodide only or octyl bromide and thereafter methyl iodide. The alternating multilayer systems were formed by consecutive adsorption of the novel polycations with up to 50% zwitterionic groups and of poly(styrenesulfonate) as the polyanion. Due to its negative charge, enzyme CDH was also firmly embedded as a polyanionic layer in the multilayer system. This LbL coating procedure was first performed on prefunctionalized silicon wafers and studied in detail with ellipsometry as well as contact angle (CA) and zetapotential (ZP) measurements before it was transferred to prefunctionalized PDMS and analyzed by CA and ZP measurements as well as atomic force microscopy. The coatings comprising six layers were stable and yielded a more neutral and hydrophilic surface than did PDMS, the polycation with 50% zwitterionic groups having the largest effect. Enzyme activity was found to be dependent on the depth of embedment in the multilayer coating. Depending on the used polymeric building block, up to a 60% reduction in the amount of adhering bacteria and clear evidence for killed bacteria due to the antimicrobial functionality of the coating could be confirmed. Overall, this work demonstrates the feasibility of an easy to perform and shape-independent method for preparing an antifouling and antimicrobial coating for the significant reduction of biofilm formation and thus reducing the risk of acquiring infections by using urinary catheters.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Citations
Cites
Banerjee, I., Pangule, R. C., & Kane, R. S. (2010) · Advanced Materials · doi:10.1002/adma.201001215 · Cited by 2,795
Atom Transfer Radical Polymerization (ATRP): Current Status and Future Perspectives
Matyjaszewski, K. A. (2012) · Macromolecules · doi:10.1021/ma3001719 · Cited by 2,660
pH-Dependent Thickness Behavior of Sequentially Adsorbed Layers of Weak Polyelectrolytes
Shiratori, S. S., & Rubner, M. F. (2000) · Macromolecules · doi:10.1021/ma991645q · Cited by 1,591
Vrijenhoek, E. M., Hong, S., & Elimelech, M. (2001) · Journal of Membrane Science · doi:10.1016/s0376-7388(01)00376-3 · Cited by 1,208
Factors Controlling the Growth of Polyelectrolyte Multilayers
Dubas, S. T., & Schlenoff, J. B. (1999) · Macromolecules · doi:10.1021/ma981927a · Cited by 976
Jacobsen, S. M., Stickler, D. J., Mobley, H. L. T., & Shirtliff, M. E. (2008) · Clinical Microbiology Reviews · doi:10.1128/cmr.00019-07 · Cited by 853
Engineering out the Risk of Infection with Urinary Catheters
Maki, D. G., & Tambyah, P. A. (2001) · Emerging infectious diseases · doi:10.3201/eid0702.010240 · Cited by 755
Antimicrobial Polymers in Solution and on Surfaces: Overview and Functional Principles
Siedenbiedel, F., & Tiller, J. C. (2012) · Polymers · doi:10.3390/polym4010046 · Cited by 744
Integrated Antimicrobial and Nonfouling Zwitterionic Polymers
Mi, L., & Jiang, S. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201304060 · Cited by 636
Lösche, M., Schmitt, J., Decher, G., Bouwman, W. G., & Kjær, K. (1998) · Macromolecules · doi:10.1021/ma980910p · Cited by 587
Lavall�e, P., Gergely, C., Cuisinier, F., Decher, G., Schaaf, P., Voegel, J., & Picart, C. (2002) · Macromolecules · doi:10.1021/ma0119833 · Cited by 532
Zwitterionic carboxybetaine polymer surfaces and their resistance to long-term biofilm formation
Cheng, G., Li, G., Xue, H., Chen, S., Bryers, J. D., & Jiang, S. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.05.058 · Cited by 523
Formation and Stability of Multilayers of Polyelectrolytes
Hoogeveen, N. G., Stuart, M. A. C., Fleer, G. J., & Böhmer, M. R. (1996) · Langmuir · doi:10.1021/la951574y · Cited by 411
A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities
Cheng, G., Xue, H., Zhang, Z., Chen, S., & Jiang, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200803570 · Cited by 371
Antimicrobial enzymes: An emerging strategy to fight microbes and microbial biofilms
Thallinger, B., Prasetyo, E. N., Nyanhongo, G. S., & Guebitz, G. M. (2013) · Biotechnology Journal · doi:10.1002/biot.201200313 · Cited by 350
Self-citationZheng, J., Li, L., Tsao, H., Sheng, Y., Chen, S., & Jiang, S. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.059428 · Cited by 345
Permanently microbicidal materials coatings
Klibanov, A. M. (2007) · Journal of Materials Chemistry · doi:10.1039/b702079a · Cited by 330
Surpassing nature: rational design of sterile-surface materials
Lewis, K., & Klibanov, A. M. (2005) · Trends in biotechnology · doi:10.1016/j.tibtech.2005.05.004 · Cited by 310
Ion Transport and Equilibria in Polyelectrolyte Multilayers
Farhat, T. R., & Schlenoff, J. B. (2001) · Langmuir · doi:10.1021/la001298+ · Cited by 291
Schoeler, B., Kumaraswamy, G., & Caruso, F. S. (2001) · Macromolecules · doi:10.1021/ma011349p · Cited by 258
Cai, K., Rechtenbach, A., Hao, J., Bossert, J., & Jandt, K. D. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.03.020 · Cited by 255
Ruths, J., Essler, F., Decher, G., & Riegler, H. E. (2000) · Langmuir · doi:10.1021/la000257a · Cited by 250
Sui, Z., Salloum, D. S., & Schlenoff, J. B. (2003) · Langmuir · doi:10.1021/la026531d · Cited by 249
Solution-Phase Surface Modification in Intact Poly(dimethylsiloxane) Microfluidic Channels
Sui, G., Wang, J., Lee, C.-C., Lu, W., Lee, S. P., Leyton, J. V., Wu, A. M., & Tseng, H. (2006) · Analytical Chemistry · doi:10.1021/ac060605z · Cited by 238
Petri, D. F. S., Wenz, G., Schunk, P., & Schimmel, T. (1999) · Langmuir · doi:10.1021/la981379u · Cited by 238
Steitz, R., Jaeger, W., & von Klitzing, R. (2001) · Langmuir · doi:10.1021/la010168d · Cited by 235
Bauer, W. C., Fischlechner, M., Abell, C., & Huck, W. T. S. (2010) · Lab on a Chip · doi:10.1039/c004046k · Cited by 227
The role of surfaces in catheter-associated infections
Noimark, S., Dunnill, C. W., Wilson, M., & Parkin, I. P. (2009) · Chemical Society Reviews · doi:10.1039/b908260c · Cited by 216
Hydration and internal properties of polyelectrolyte multilayers
Schönhoff, M., Ball, V., Bausch, A. R., Déjugnat, C., Delorme, N., Glinel, K., von Klitzing, R., & Steitz, R. (2007) · Colloids and Surfaces A Physicochemical and Engineering Aspects · doi:10.1016/j.colsurfa.2007.02.054 · Cited by 192
Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications
Ludwig, R., Harreither, W., Tasca, F., & Gorton, L. G. O. (2010) · ChemPhysChem · doi:10.1002/cphc.201000216 · Cited by 191
Glinel, K., Moussa, A., Jonas, A. M., & Laschewsky, A. (2002) · Langmuir · doi:10.1021/la0113670 · Cited by 183
Non-leaching surfaces capable of killing microorganisms on contact
Ferreira, L., & Zumbuehl, A. (2009) · Journal of Materials Chemistry · doi:10.1039/b905668h · Cited by 174
Water and Ion Pairing in Polyelectrolyte Multilayers
Farhat, T. R., Yassin, G. H., Dubas, S. T., & Schlenoff, J. B. (1999) · Langmuir · doi:10.1021/la990631a · Cited by 173
New strategies to prevent catheter-associated urinary tract infections
Siddiq, D. M., & Darouiche, R. O. (2012) · Nature Reviews Urology · doi:10.1038/nrurol.2012.68 · Cited by 170
Shao, Q., Mi, L., Han, X., Bai, T., Liu, S., Li, Y., & Jiang, S. (2014) · The Journal of Physical Chemistry B · doi:10.1021/jp503473u · Cited by 170
Li, X., Li, P., Saravanan, R., Basu, A., Mishra, B., Lim, S. H., Su, X., Tambyah, P. A., & Leong, S. S. J. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.09.009 · Cited by 152
Modified Calcite Deposition Due to Ultrathin Organic Films on Silicon Substrates
Archibald, D. D., Qadri, S. B., & Gaber, B. P. (1996) · Langmuir · doi:10.1021/la950330a · Cited by 143
Influence of Charged Groups on the Properties of Zwitterionic Moieties: A Molecular Simulation Study
Shao, Q., & Jiang, S. (2014) · The Journal of Physical Chemistry B · doi:10.1021/jp5027114 · Cited by 142
Blanco, C. D., Ortner, A., Dimitrov, R., Navarro, A., Mendoza, E., & Tzanov, T. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am501961b · Cited by 137
Topal, J. E., Conklin, S., Camp, K., Morris, V., Balcezak, T. J., & Herbert, P. N. (2005) · American Journal of Medical Quality · doi:10.1177/1062860605276074 · Cited by 136
Dodoo, S., Steitz, R., Laschewsky, A., & von Klitzing, R. (2011) · Physical Chemistry Chemical Physics · doi:10.1039/c0cp01357a · Cited by 115
Synthesis and Properties of Low-Polydispersity Poly(sulfopropylbetaine)s and Their Block Copolymers
Lowe, A. B., Billingham, N. Ć., & Armes, S. P. (1999) · Macromolecules · doi:10.1021/ma980543h · Cited by 111
Polyelectrolyte Adsorption on Oxides
Hoogeveen, N. G., Stuart, M. A. C., & Fleer, G. J. (1996) · Journal of Colloid and Interface Science · doi:10.1006/jcis.1996.0445 · Cited by 110
Keefe, A. J., Brault, N. D., & Jiang, S. (2012) · Biomacromolecules · doi:10.1021/bm300399s · Cited by 108
Cross-Linked Polyelectrolyte Multilayers for Marine Antifouling Applications
Zhu, X., Jańczewski, D., Lee, S. S. C., Teo, S. L., & Vancso, G. J. (2013) · ACS Applied Materials & Interfaces · doi:10.1021/am4015549 · Cited by 106
Yang, Q., & Ulbricht, M. (2012) · Chemistry of Materials · doi:10.1021/cm301116p · Cited by 97
Self-citationXuan, F., & Liu, J. (2009) · Polymer International · doi:10.1002/pi.2679 · Cited by 89
Molecular mass dependence of adsorbed amount and hydrodynamic thickness of polyelectrolyte layers
Seyrek, E., Osorio, J. M. H., Sadeghpour, A., Szilágyi, I., & Borkovec, M. (2011) · Physical Chemistry Chemical Physics · doi:10.1039/c1cp20654k · Cited by 67
Flitsch, A., Prasetyo, E. N., Sygmund, C., Ludwig, R., Nyanhongo, G. S., & Guebitz, G. M. (2012) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2012.10.007 · Cited by 55
Self-citationLi, X., Cao, Y., Kang, G., Yu, H., Jie, X., & Yuan, Q. (2014) · Journal of Applied Polymer Science · doi:10.1002/app.41144 · Cited by 55
Regev-Shoshani, G., Ko, M., Crowe, A., & Av-Gay, Y. (2011) · Urology · doi:10.1016/j.urology.2011.02.063 · Cited by 50
Barford, J. M. T., Anson, K. M., Hu, Y., & Coates, A. R. M. (2008) · British Journal of Urology · doi:10.1111/j.1464-410x.2008.07465.x · Cited by 47
Lopez, A. I., Kumar, A., Planas, M. R., Li, Y., Nguyen, T. V., & Cai, C. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.02.056 · Cited by 45
Lee, W., & Chen, C.-F. (1998) · Journal of Applied Polymer Science · doi:10.1002/(sici)1097-4628(19980906)69:10 · Cited by 45
Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system
Thallinger, B., Brandauer, M., Burger, P., Sygmund, C., Ludwig, R., Ivanova, K., Kun, J., Scaini, D., Burnet, M. W. M., Tzanov, T., et al. (2015) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33491 · Cited by 44
Self-citationDemming, S., Lesche, C., Schmolke, H., Klages, C. ‐., & Büttgenbach, S. (2011) · physica status solidi (a) · doi:10.1002/pssa.201000967 · Cited by 35
Ultrasound coating of polydimethylsiloxanes with antimicrobial enzymes
Lipovsky, A., Thallinger, B., Perelshtein, I., Ludwig, R., Sygmund, C., Nyanhongo, G. S., Guebitz, G. M., & Gedanken, A. (2015) · Journal of Materials Chemistry B · doi:10.1039/c5tb00671f · Cited by 30
Self-citationEngineering of Pyranose Dehydrogenase for Increased Oxygen Reactivity
Krondorfer, I., Lipp, K., Brugger, D., Staudigl, P., Sygmund, C., Haltrich, D., & Peterbauer, C. (2014) · PLoS ONE · doi:10.1371/journal.pone.0091145 · Cited by 20
Guillaume-Gentil, O. J., Zahn, R., Lindhoud, S., Graf, N., Vörös, J., & Zambelli, T. (2011) · Soft Matter · doi:10.1039/c0sm01451f · Cited by 20
Berndt, E., Behnke, S., & Ulbricht, M. (2011) · European Polymer Journal · doi:10.1016/j.eurpolymj.2011.09.019 · Cited by 12
Self-citation
Cited by
Sorted by citations
Eduok, U. M., Faye, O., & Szpunar, J. A. (2017) · Progress in Organic Coatings · doi:10.1016/j.porgcoat.2017.05.012 · Cited by 665
Jiao, Y., Niu, L.-N., Ma, S., Li, J., Tay, F. R., & Chen, J.-H. (2017) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2017.03.001 · Cited by 639
Synthesis, Assembly, and Applications of Hybrid Nanostructures for Biosensing
Zhang, S., Geryak, R. D., Geldmeier, J. A., Kim, S., & Tsukruk, V. V. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00088 · Cited by 341
Antimicrobial and Antifouling Strategies for Polymeric Medical Devices
Zander, Z. K., & Becker, M. L. (2017) · ACS Macro Letters · doi:10.1021/acsmacrolett.7b00879 · Cited by 312
Biomimetic polymeric superhydrophobic surfaces and nanostructures: from fabrication to applications
Wen, G., Guo, Z., & Liu, W. (2017) · Nanoscale · doi:10.1039/c7nr00096k · Cited by 293
Metadata
- Authors
- Vaterrodt, A., Thallinger, B., Daumann, K., Koch, D., Guebitz, G. M., & Ulbricht, M.
- Affiliations
- University of Duisburg-Essen (DE)
- BOKU University (AT)
- Austrian Centre of Industrial Biotechnology (Austria) (AT)
- Corresponding author
- Ulbricht, Mathias
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-01-26
- Volume
- 32
- Issue
- 5
- Pages
- 1347-1359
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2234833147
- Cited by
- 150
- References
- 60
- Citations by year
- 2026: 8 · 2025: 5 · 2024: 10 · 2023: 12 · 2022: 16 · 2021: 14 · 2020: 18 · 2019: 14 · 2018: 22 · 2017: 21 · 2016: 10
- FWCI
- 9.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Langmuir (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bundesministerium f?r Wissenschaft, Forschung und Wirtschaft
- Bundesministerium f?r Verkehr, Innovation und Technologie
- ?sterreichische Forschungsf?rderungsgesellschaft
- Seventh Framework Programme (FP7-27840)
- Standortagentur Tirol
- Government of Lower Austria
- Steirische Wirtschaftsf?rderungsgesellschaft mbH SFG
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Keywords
- antibacterial coatings, polyzwitterions, layer-by-layer assembly, cellobiose dehydrogenase, biofilm inhibition, catheter-associated urinary tract infection, zwitterionic polymers, quaternary ammonium polymers, atom transfer radical polymerization, polystyrene sulfonate, surface hydrophilicity, zeta potential
- MeSH terms
- Quaternary Ammonium Compounds, Anti-Bacterial Agents, Bacterial Adhesion, Carbohydrate Dehydrogenases, Dimethylpolysiloxanes, Freeze Drying, Hydrogen Peroxide, Methacrylates, Nylons, Polystyrenes, Silicon, Staphylococcus aureus, Biofilms, Sodium Azide, Electrochemical Techniques, Photoelectron Spectroscopy, Urinary Catheters
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
