Continuous formation of ultrathin, strong collagen sheets with tunable anisotropy and compaction
Open accessAbstract
The multiscale organization of protein-based fibrillar materials is a hallmark of many organs, but the recapitulation of hierarchal structures down to fibrillar scales, which is a requirement for withstanding physiological loading forces, has been challenging. We present a microfluidic strategy for the continuous, large-scale formation of strong, handleable, free-standing, multicentimeter-wide collagen sheets of unprecedented thinness through the application of hydrodynamic focusing with the simultaneous imposition of strain. Sheets as thin as 1.9 μm displayed tensile strengths of 0.5-2.7 MPa, Young's moduli of 3-36 MPa, and modulated the diffusion of molecules as a function of collagen nanoscale structure. Smooth muscle cells cultured on engineered sheets oriented in the direction of aligned collagen fibrils and generated coordinated vasomotor responses. The described biofabrication approach enables rapid formation of ultrathin collagen sheets that withstand physiologically relevant loads for applications in tissue engineering and regenerative medicine, as well as in organ-on-chip and biohybrid devices.
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Electrospinning or 3D microextrusion printing enable production of highly aligned collagen sheets or hydrogels with spaces estimated 2-20 μm between aligned fibrils.
“Furthermore, techniques such as electrospinning or 3D microextrusion printing enable the production of highly aligned collagen sheets, or hydrogels with spaces an estimated 2-20 μm between aligned fibrils [34,35].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [34]
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References 65 · Cited by 34
- Authors
- Malladi, S., Miranda-Nieves, D., Leng, L., Grainger, S. J., Tarabanis, C., Nesmith, A. P., Kosaraju, R., Haller, C. A., Parker, K. K., Chaikof, E. L., & Günther, A.
- Affiliations
- University of Toronto (CA)
- Beth Israel Deaconess Medical Center (US)
- Harvard University (US)
- University of Toronto , , , ,
- Corresponding author
- Chaikof, Elliot L.
- ORCID
- Venue
- ACS Biomaterials Science & Engineering
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-05-26
- Volume
- 6
- Issue
- 7
- Pages
- 4236-4246
- ISSN
- 2373-9878 (ISSN-L)
- PMC
- PMC7362332
- OpenAlex
- W3031901586
- Cited by
- 34
- References
- 65
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 6 · 2021: 6 · 2020: 2
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- ACS Biomaterials Science & Engineering (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (1-UG3-HL141798-01, RO1HL083867, RO1HL60464, RO1HL71336)
- Division of Materials Research (DMR-1420570)
- National Center for Advancing Translational Sciences (UH3TR000522)
- Natural Sciences and Engineering Research Council of Canada (RGPIN-2017-06781)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- collagen sheet, hydrodynamic focusing, collagen fibril alignment, anisotropy, tensile strength, Young's modulus, smooth muscle cells, vasomotor response, organ-on-a-chip, biohybrid devices
- MeSH terms
- Collagen, Extracellular Matrix, Tensile Strength, Anisotropy, Tissue Engineering
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