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Cited by 15Bacterial Genetics and BiotechnologyInfective Endocarditis Diagnosis and ManagementMetalloenzymes and iron-sulfur proteins

Intracellular Metal Speciation in Streptococcus sanguinis Establishes SsaACB as Critical for Redox Maintenance

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Murgas, Cody J., Green, Shannon P., Forney, Ashley K., Korba, Rachel M., An, Seon-Sook, Kitten, Todd, Lucas, Heather R.·2020·ACS Infectious Diseases
DOI 10.1021/acsinfecdis.0c00132PMC PMC7452790
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Abstract

Streptococcus sanguinis is an oral commensal bacterium, but it can colonize pre-existing heart valve vegetations if introduced into the bloodstream, leading to infective endocarditis. Loss of Mn- or Fe-cofactored virulence determinants are thought to result in weakening of this bacterium. Indeed, intracellular Mn accumulation mediated by the lipoprotein SsaB, a component of the SsaACB transporter complex, has been shown to promote virulence for endocarditis and O2 tolerance. To delineate intracellular metal-ion abundance and redox speciation within S. sanguinis, we developed a protocol exploiting two spectroscopic techniques, Inductively coupled plasma-optical emission spectrometry (ICP-OES) and electron paramagnetic resonance (EPR) spectroscopy, to respectively quantify total intracellular metal concentrations and directly measure redox speciation of Fe and Mn within intact whole-cell samples. Addition of the cell-permeable siderophore deferoxamine shifts the oxidation states of accessible Fe and Mn from reduced-to-oxidized, as verified by magnetic moment calculations, aiding in the characterization of intracellular metal pools and metal sequestration levels for Mn2+ and Fe. We have applied this methodology to S. sanguinis and an SsaACB knockout strain (ΔssaACB), indicating that SsaACB mediates both Mn and Fe uptake, directly influencing the metal-ion pools available for biological inorganic pathways. Mn supplementation of ΔssaACB returns total intracellular Mn to wild-type levels, but it does not restore wild-type redox speciation or distribution of metal cofactor availability for either Mn or Fe. Our results highlight the biochemical basis for S. sanguinis oxidative resistance, revealing a dynamic role for SsaACB in controlling redox homeostasis by managing the intracellular Fe/Mn composition and distribution.

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  • Manganese coordination environments in cells have been probed using EPR-based techniques that take advantage of Mn2+ being high-spin with a unique EPR signature.

    “Manganese coordination environments in cells have been probed using electron paramagnetic resonance (EPR)-based techniques that take advantage of the fact that Mn2+ is high-spin with a unique EPR signature.112,113”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [112]

  • Quantitation of this quenching suggests that roughly one-half of intracellular Mn2+ is in a labile pool accessible to deferoxamine.

    “Quantitation of this quenching suggests that roughly one-half of intracellular Mn2+ is in a labile pool accessible to deferoxamine.112”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [112]

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Cited by

Sorted by citations

Authors
Murgas, C. J., Green, S. P., Forney, A. K., Korba, R. M., An, S.-S., Kitten, T., & Lucas, H. R.
Affiliations
Virginia Commonwealth University (US)
Corresponding author
  • Kitten, Todd
  • Lucas, Heather R.
ORCID
Venue
ACS Infectious Diseases
Publisher
American Chemical Society (ACS)
Published
2020-04-24
Volume
6
Issue
7
Pages
1906-1921
ISSN
2373-8227 (ISSN-L)
DOI
10.1021/acsinfecdis.0c00132
PMC
PMC7452790
OpenAlex
W3020215973
Cited by
15
References
2
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2
FWCI
1.30
Type
journal article
Language
en
Open access
Licence
Locations
  • ACS Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Allergy and Infectious Diseases (R01AI114926)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Epidemiology › Infective Endocarditis Diagnosis and Management
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
Keywords
Streptococcus sanguinis, manganese accumulation, iron oxidation state, redox homeostasis, deferoxamine, oxidation resistance, infective endocarditis, metal cofactors, metal uptake, oral commensal bacteria
MeSH terms
Iron, Oxidation-Reduction, Streptococcus sanguis, Virulence, Virulence Factors

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